biostudies-literatureSimons LMIntramural NIH HHS3367-3379https://www.ebi.ac.uk/biostudies/studies/S-EPMC10334573biostudies-literatureUnknown41(21)Recent work demonstrating that asymptomatic carriers of P. falciparum parasites make up a large part of the infectious reservoir highlights the need for an effective malaria vaccine. Given the historical challenges of vaccine development, multiple parasite stages have been targeted, including the sexual stages required for transmission. Using flow cytometry to efficiently screen for P. falciparum gamete/zygote surface reactivity, we identified 82 antibodies that bound live P. falciparum gametes/zygotes. Ten antibodies had significant transmission-reducing activity (TRA) in a standard membrane feeding assay and were subcloned along with 9 nonTRA antibodies as comparators. After subcloning, only eight of the monoclonals obtained have significant TRA. These eight TRA mAbs do not recognize epiVaccineExtending the range of Plasmodium falciparum transmission blocking antibodies.PMC10334573Z01 AI000947Herrera RLee SMDuffy PEMiura KUnderwood KAmoah LCanepa GNarum DLAcquah FBarillas-Mury CLocke EGombakomba NWilliamson KCFerrer PSimons LMLong CfalseExtending the range of Plasmodium falciparum transmission blocking antibodies.Recent work demonstrating that asymptomatic carriers of P. falciparum parasites make up a large part of the infectious reservoir highlights the need for an effective malaria vaccine. Given the historical challenges of vaccine development, multiple parasite stages have been targeted, including the sexual stages required for transmission. Using flow cytometry to efficiently screen for P. falciparum gamete/zygote surface reactivity, we identified 82 antibodies that bound live P. falciparum gametes/zygotes. Ten antibodies had significant transmission-reducing activity (TRA) in a standard membrane feeding assay and were subcloned along with 9 nonTRA antibodies as comparators. After subcloning, only eight of the monoclonals obtained have significant TRA. These eight TRA mAbs do not recognize epi2023-01-01T00:00:00Z2023 May2026-06-01T18:58:05.786Z2026-05-19T03:07:31.18ZS-EPMC103345733710072110.1016/j.vaccine.2023.04.042