{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Singh AK"],"funding":["Narodowe Centrum Nauki"],"pagination":["125"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10334657"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["22(1)"],"pubmed_abstract":["<h4>Background</h4>Mammalian metallothioneins (MTs) are small (6-7 kDa), intracellular, cysteine-rich, metal-binding proteins involved, inter alia, in the homeostasis of zinc and copper, detoxification of heavy metals, antioxidation against reactive oxygen species, and protection against DNA damage. The high cysteine content (~ 30%) in MTs makes them toxic to bacterial cells during protein production, resulting in low yield. To address this issue, we present for the first time a combinatorial approach using the small ubiquitin-like modifier (SUMO) and/or sortase as fusion tags for high-level expression of human MT3 in E. coli and its purification by three different strategies.<h4>Results</h4>Three different plasmids were generated using SUMO, sortase A pentamutant (eSrtA), and sortase reco"],"journal":["Microbial cell factories"],"pubmed_title":["Calcium-assisted sortase A cleavage of SUMOylated metallothionein constructs leads to high-yield production of human MT3."],"pmcid":["PMC10334657"],"funding_grant_id":["2019/33/B/ST4/02428"],"pubmed_authors":["Singh AK","Krezel A"],"additional_accession":[]},"is_claimable":false,"name":"Calcium-assisted sortase A cleavage of SUMOylated metallothionein constructs leads to high-yield production of human MT3.","description":"<h4>Background</h4>Mammalian metallothioneins (MTs) are small (6-7 kDa), intracellular, cysteine-rich, metal-binding proteins involved, inter alia, in the homeostasis of zinc and copper, detoxification of heavy metals, antioxidation against reactive oxygen species, and protection against DNA damage. The high cysteine content (~ 30%) in MTs makes them toxic to bacterial cells during protein production, resulting in low yield. To address this issue, we present for the first time a combinatorial approach using the small ubiquitin-like modifier (SUMO) and/or sortase as fusion tags for high-level expression of human MT3 in E. coli and its purification by three different strategies.<h4>Results</h4>Three different plasmids were generated using SUMO, sortase A pentamutant (eSrtA), and sortase reco","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jul","modification":"2025-04-22T21:11:45.115Z","creation":"2025-04-22T21:11:45.115Z"},"accession":"S-EPMC10334657","cross_references":{"pubmed":["37434134"],"doi":["10.1186/s12934-023-02134-x"]}}