biostudies-literatureUnknown33Abate MNanodiamonds are innovative nanocrystalline carbon particles able to deliver chemically conjugated miRNAs. In oncology, the use of miRNA-based therapies may represent an advantage, based on their ability to simultaneously target multiple intracellular oncogenic targets. Here, nanodiamonds were tested and optimized to deliver miR-34a, a miRNA playing a key role in inhibiting tumor development and progression in many cancers. The physical-chemical properties of nanodiamonds were investigated suggesting electrical stability and uniformity of structure and size. Moreover, we evaluated nanodiamond cytotoxicity on two breast cancer cell models and confirmed their excellent biocompatibility. Subsequently, nanodiamonds were conjugated with miR-34a, using the chemical crosslinker polyethyleneimine;Molecular therapy. Nucleic acids127-141https://www.ebi.ac.uk/biostudies/studies/S-EPMC10336355biostudies-literatureFluorescent nanodiamonds as innovative delivery systems for MiR-34a replacement in breast cancer.PMC10336355Graziano SFAbate MLuce AAmler EScrima MZappavigna SFranco RMisso GLeonetti CSperlongano RMarino FZLombardi ACardile FCaraglia MRonchi ACampani VPorru MDe Rosa GNele VBocchetti MAlfano RfalseFluorescent nanodiamonds as innovative delivery systems for MiR-34a replacement in breast cancer.Nanodiamonds are innovative nanocrystalline carbon particles able to deliver chemically conjugated miRNAs. In oncology, the use of miRNA-based therapies may represent an advantage, based on their ability to simultaneously target multiple intracellular oncogenic targets. Here, nanodiamonds were tested and optimized to deliver miR-34a, a miRNA playing a key role in inhibiting tumor development and progression in many cancers. The physical-chemical properties of nanodiamonds were investigated suggesting electrical stability and uniformity of structure and size. Moreover, we evaluated nanodiamond cytotoxicity on two breast cancer cell models and confirmed their excellent biocompatibility. Subsequently, nanodiamonds were conjugated with miR-34a, using the chemical crosslinker polyethyleneimine;2023-01-01T00:00:00Z2023 Sep2025-04-04T13:51:36.898Z2025-04-04T13:51:36.898ZS-EPMC103363553744904210.1016/j.omtn.2023.06.012