biostudies-literatureNienhuser HDeutsche ForschungsgemeinschaftNIDDK NIH HHSFoundation for the National Institutes of HealthDeutsche KrebshilfeNCI NIH HHS654-665https://www.ebi.ac.uk/biostudies/studies/S-EPMC10337481biostudies-literatureUnknown70(4)<h4>Background and aims</h4>The gastric epithelium undergoes continuous turnover. Corpus epithelial stem cells located in the gastric isthmus serve as a source of tissue self-renewal. We recently identified the transcription factor Mist1 as a marker for this corpus stem cell population that can give rise to cancer. The aim here was to investigate the regulation of the Mist1+ stem cells in the response to gastric injury and inflammation.<h4>Methods</h4>We used Mist1CreERT;R26-Tdtomato mice in two models of injury and inflammation: the acetic acid-induced ulcer and infection with <i>Helicobacter felis</i>. We analysed lineage tracing at both early (7 to 30 days) and late (30 to 90 days) time points. Mist1CreERT;R26-Tdtomato;Lgr5DTR-eGFP mice were used to ablate the corpus basal Lgr5+ cell poGutMist1+ gastric isthmus stem cells are regulated by Wnt5a and expand in response to injury and inflammation in mice.PMC103374815UO1DK103155R35 CA210088NI1810/1-1R01 CA224428R37 DK052778U01 DK103155U01 DK08553270111870R35CA210088U24 DK085532P30 CA0136965R37DK052778Kim WMiddelhoff MNienhuser HValenti GMalagola EDer CJBass AWang TCRuan THayakawa YfalseMist1+ gastric isthmus stem cells are regulated by Wnt5a and expand in response to injury and inflammation in mice.<h4>Background and aims</h4>The gastric epithelium undergoes continuous turnover. Corpus epithelial stem cells located in the gastric isthmus serve as a source of tissue self-renewal. We recently identified the transcription factor Mist1 as a marker for this corpus stem cell population that can give rise to cancer. The aim here was to investigate the regulation of the Mist1+ stem cells in the response to gastric injury and inflammation.<h4>Methods</h4>We used Mist1CreERT;R26-Tdtomato mice in two models of injury and inflammation: the acetic acid-induced ulcer and infection with <i>Helicobacter felis</i>. We analysed lineage tracing at both early (7 to 30 days) and late (30 to 90 days) time points. Mist1CreERT;R26-Tdtomato;Lgr5DTR-eGFP mice were used to ablate the corpus basal Lgr5+ cell po2021-01-01T00:00:00Z2021 Apr2025-04-04T13:52:35.861Z2025-04-04T13:52:35.861ZS-EPMC103374813270961310.1136/gutjnl-2020-320742