biostudies-literatureIgarashi KJCancer Research UKNIDDK NIH HHSNHLBI NIH HHSMedical Research CouncilNCI NIH HHSWellcome Trust3366-3377https://www.ebi.ac.uk/biostudies/studies/S-EPMC10345863biostudies-literatureUnknown7(14)Hematopoietic stem cells (HSCs) are a rare type of hematopoietic cell that can entirely reconstitute the blood and immune system after transplantation. Allogeneic HSC transplantation (HSCT) is used clinically as a curative therapy for a range of hematolymphoid diseases; however, it remains a high-risk therapy because of its potential side effects, including poor graft function and graft-versus-host disease (GVHD). Ex vivo HSC expansion has been suggested as an approach to improve hematopoietic reconstitution in low-cell dose grafts. Here, we demonstrate that the selectivity of polyvinyl alcohol (PVA)-based mouse HSC cultures can be improved using physioxic culture conditions. Single-cell transcriptomic analysis helped confirm the inhibition of lineage-committed progenitor cells in physioxiBlood advancesPhysioxia improves the selectivity of hematopoietic stem cell expansion cultures.PMC10345863T32 CA196585R01 DK116944R01 HL147124K99 HL150218MC_UU_00016/18Igarashi KJGottgens BCzechowicz ABridge KSHo PYChan YYKealy DHickey JWWilkinson ACKhoo HMNakauchi HKucinski INolan GPTan TKBhadury JNiizuma KHsu IfalsePhysioxia improves the selectivity of hematopoietic stem cell expansion cultures.Hematopoietic stem cells (HSCs) are a rare type of hematopoietic cell that can entirely reconstitute the blood and immune system after transplantation. Allogeneic HSC transplantation (HSCT) is used clinically as a curative therapy for a range of hematolymphoid diseases; however, it remains a high-risk therapy because of its potential side effects, including poor graft function and graft-versus-host disease (GVHD). Ex vivo HSC expansion has been suggested as an approach to improve hematopoietic reconstitution in low-cell dose grafts. Here, we demonstrate that the selectivity of polyvinyl alcohol (PVA)-based mouse HSC cultures can be improved using physioxic culture conditions. Single-cell transcriptomic analysis helped confirm the inhibition of lineage-committed progenitor cells in physioxi2023-01-01T00:00:00Z2023 Jul2026-06-02T12:03:29.193Z2026-04-18T03:11:18.446ZS-EPMC103458633680978110.1182/bloodadvances.2023009668