biostudies-literatureUnknownBhuiyan SANEI NIH HHSNICHD NIH HHSNIDA NIH HHSNINDS NIH HHSPeripheral sensory neurons in the dorsal root ganglion (DRG) and trigeminal ganglion (TG) are specialized to detect and transduce diverse environmental stimuli including touch, temperature, and pain to the central nervous system. Recent advances in single-cell RNA-sequencing (scRNA-seq) have provided new insights into the diversity of sensory ganglia cell types in rodents, non-human primates, and humans, but it remains difficult to compare transcriptomically defined cell types across studies and species. Here, we built cross-species harmonized atlases of DRG and TG cell types that describe 18 neuronal and 11 non-neuronal cell types across 6 species and 19 studies. We then demonstrate the utility of this harmonized reference atlas by using it to annotate newly profiled DRG nuclei/cells from both human and the highly regenerative axolotl. We observe that the transcriptomic profiles of sensory neuron subtypes are broadly similar across vertebrates, but the expression of functionally important neuropeptides and channels can vary notably. The new resources and data presented here can guide future studies in comparative transcriptomics, simplify cell type nomenclature differences across studies, and help prioritize targets for future pain therapy development.bioRxiv : the preprint server for biology2023.07.04.547740https://www.ebi.ac.uk/biostudies/studies/S-EPMC10350076biostudies-literatureHarmonized cross-species cell atlases of trigeminal and dorsal root ganglia.PMC10350076U19 NS130608U01 EY034709U19 NS130607U19 NS130617K08 NS101064R01 NS119476P50 HD105351DP1 DA054343Slivicki RASankaranarayanan ITochitsky ISemizoglou EBhatia PCopits BAWoolf CJJain ATavares-Ferreira DBertels ZBhuiyan SAPantaleo KIRenthal WGereau RWWhited JLXu MYang LMcIlvried LADel Rosario JSYi JCat VErdogan BWidman AJGreen ULennerz JKMwirigi JMBlair SPrice TJfalseHarmonized cross-species cell atlases of trigeminal and dorsal root ganglia.Peripheral sensory neurons in the dorsal root ganglion (DRG) and trigeminal ganglion (TG) are specialized to detect and transduce diverse environmental stimuli including touch, temperature, and pain to the central nervous system. Recent advances in single-cell RNA-sequencing (scRNA-seq) have provided new insights into the diversity of sensory ganglia cell types in rodents, non-human primates, and humans, but it remains difficult to compare transcriptomically defined cell types across studies and species. Here, we built cross-species harmonized atlases of DRG and TG cell types that describe 18 neuronal and 11 non-neuronal cell types across 6 species and 19 studies. We then demonstrate the utility of this harmonized reference atlas by using it to annotate newly profiled DRG nuclei/cells from both human and the highly regenerative axolotl. We observe that the transcriptomic profiles of sensory neuron subtypes are broadly similar across vertebrates, but the expression of functionally important neuropeptides and channels can vary notably. The new resources and data presented here can guide future studies in comparative transcriptomics, simplify cell type nomenclature differences across studies, and help prioritize targets for future pain therapy development.2023-01-01T00:00:00Z2023 Jul2025-08-15T03:06:56.204Z2025-04-04T10:32:46.158ZS-EPMC103500763746173610.1101/2023.07.04.547740