biostudies-literatureUnknown14Ouyang KPineapple [<i>Ananas comosus</i> (L.) Merr.] is the most economically important crop possessing crassulacean acid metabolism (CAM) photosynthesis which has a higher water use efficiency by control of nocturnal opening and diurnal closure of stomata. To provide novel insights into the diel regulatory landscape in pineapple leaves, we performed genome-wide mapping of DNase I hypersensitive sites (DHSs) in pineapple leaves at day (2a.m.) and night (10a.m.) using a simplified DNase-seq method. As a result, totally 33340 and 28753 DHSs were found in green-tip tissue, and 29597 and 40068 were identified in white-base tissue at 2a.m. and 10a.m., respectively. We observed that majority of the pineapple genes occupied less than two DHSs with length shorter than 1 kb, and the promotor DHSs showed a Frontiers in genetics1086554https://www.ebi.ac.uk/biostudies/studies/S-EPMC10352800biostudies-literatureGenome-wide mapping of DNase I hypersensitive sites in pineapple leaves.PMC10352800Li ZZhang ZOuyang KMiao LLiang QfalseGenome-wide mapping of DNase I hypersensitive sites in pineapple leaves.Pineapple [<i>Ananas comosus</i> (L.) Merr.] is the most economically important crop possessing crassulacean acid metabolism (CAM) photosynthesis which has a higher water use efficiency by control of nocturnal opening and diurnal closure of stomata. To provide novel insights into the diel regulatory landscape in pineapple leaves, we performed genome-wide mapping of DNase I hypersensitive sites (DHSs) in pineapple leaves at day (2a.m.) and night (10a.m.) using a simplified DNase-seq method. As a result, totally 33340 and 28753 DHSs were found in green-tip tissue, and 29597 and 40068 were identified in white-base tissue at 2a.m. and 10a.m., respectively. We observed that majority of the pineapple genes occupied less than two DHSs with length shorter than 1 kb, and the promotor DHSs showed a 2023-01-01T00:00:00Z20232025-04-05T14:51:34.729Z2025-04-05T14:51:34.729ZS-EPMC103528003747003610.3389/fgene.2023.1086554