{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Blandino F"],"funding":["Associazione Italiana per la Ricerca sul Cancro","Italian Ministry of Health"],"pagination":["6723-6737"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10359636"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["51(13)"],"pubmed_abstract":["The MUS81 complex is crucial for preserving genome stability through resolution of branched DNA intermediates in mitosis and also for the processing of deprotected replication forks in BRCA2-deficient cells. Because of the existence of two different MUS81 complexes in mammalian cells that act in M- or S-phase, whether and how the PARPi sensitivity of BRCA2-deficient cells is affected by loss of MUS81 function is unclear. Here, using a mutant of MUS81 that impairs its function in M-phase, we show that viability of BRCA2-deficient cells but not their PARPi sensitivity requires a fully-functional MUS81 complex in mitosis. In contrast, expression of a constitutively-active MUS81 is sufficient to confer PARPi resistance. From a mechanistic point of view, our data indicate that deregulated actio"],"journal":["Nucleic acids research"],"pubmed_title":["Phosphorylation status of MUS81 is a modifier of Olaparib sensitivity in BRCA2-deficient cells."],"pmcid":["PMC10359636"],"funding_grant_id":["19971","RF-2016-02362022","21428"],"pubmed_authors":["Noto A","Figlioli C","Pichierri P","Pugliese GM","Malacaria E","Franchitto A","Blandino F"],"additional_accession":[]},"is_claimable":false,"name":"Phosphorylation status of MUS81 is a modifier of Olaparib sensitivity in BRCA2-deficient cells.","description":"The MUS81 complex is crucial for preserving genome stability through resolution of branched DNA intermediates in mitosis and also for the processing of deprotected replication forks in BRCA2-deficient cells. Because of the existence of two different MUS81 complexes in mammalian cells that act in M- or S-phase, whether and how the PARPi sensitivity of BRCA2-deficient cells is affected by loss of MUS81 function is unclear. Here, using a mutant of MUS81 that impairs its function in M-phase, we show that viability of BRCA2-deficient cells but not their PARPi sensitivity requires a fully-functional MUS81 complex in mitosis. In contrast, expression of a constitutively-active MUS81 is sufficient to confer PARPi resistance. From a mechanistic point of view, our data indicate that deregulated actio","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jul","modification":"2025-07-02T03:04:38.228Z","creation":"2025-07-02T03:04:38.228Z"},"accession":"S-EPMC10359636","cross_references":{"pubmed":["37254810"],"doi":["10.1093/nar/gkad470"]}}