biostudies-literatureUnknown13(1)Stevanovic KSThe authors acknowledge funding provided by the Institute of Physics Belgrade, through the grant by the Ministry of Education, Science, and Technological Development of the Republic of SerbiaIon channels are vital components of filamentous fungi signaling in communication with their environment. We exploited the ability of the apical region of growing sporangiophores of Phycomyces blakesleeanus to form membrane-enveloped cytoplasmic droplets (CDs), to examine ion currents in the filamentous fungi native plasma membrane. In hypoosmotic conditions, the dominant current in the CDs is ORIC, an osmotically activated, anionic, outwardly rectified, fast inactivating instantaneous current that we have previously characterized. Here, we examined the effect of ATP on ORIC. We show that CDs contain active mitochondria, and that respiration inhibition by azide accelerates ORIC inactivation. ATP, added intracellularly, reduced ORIC run-down and shifted the voltage dependence of inactivatioScientific reports11897https://www.ebi.ac.uk/biostudies/studies/S-EPMC10366193biostudies-literatureATP modulation of osmotically activated anionic current in the membrane of Phycomyces blakesleeanus sporangiophore.PMC10366193Todorovic NVKrizak SZivic MZPajic TStevanovic KSCepkenovic BfalseATP modulation of osmotically activated anionic current in the membrane of Phycomyces blakesleeanus sporangiophore.Ion channels are vital components of filamentous fungi signaling in communication with their environment. We exploited the ability of the apical region of growing sporangiophores of Phycomyces blakesleeanus to form membrane-enveloped cytoplasmic droplets (CDs), to examine ion currents in the filamentous fungi native plasma membrane. In hypoosmotic conditions, the dominant current in the CDs is ORIC, an osmotically activated, anionic, outwardly rectified, fast inactivating instantaneous current that we have previously characterized. Here, we examined the effect of ATP on ORIC. We show that CDs contain active mitochondria, and that respiration inhibition by azide accelerates ORIC inactivation. ATP, added intracellularly, reduced ORIC run-down and shifted the voltage dependence of inactivatio2023-01-01T00:00:00Z2023 Jul2025-04-04T20:33:25.668Z2025-02-19T00:26:29.76ZS-EPMC103661933748820510.1038/s41598-023-39021-9