biostudies-literatureUnknown3Hafele MWhile it is well established that the isoform 2 of the hyperpolarization-activated cyclic nucleotide-gated cation channel (HCN2) plays an important role in the development and maintenance of pain, the role of the closely related HCN4 isoform in the processing of nociceptive signals is not known. HCN4 channels are highly expressed in the thalamus, a region important for stimulus transmission and information processing. We used a brain-specific HCN4-knockout mouse line (HCN4-KO) to explore the role of HCN4 channels in acute nociceptive processing using several behavioral tests as well as a multimodal magnetic resonance imaging (MRI) approach. Functional MRI (fMRI) brain responses were measured during acute peripheral thermal stimulation complemented by resting state (RS) before and after stiFrontiers in network physiology1090502https://www.ebi.ac.uk/biostudies/studies/S-EPMC10368246biostudies-literatureThe impact of HCN4 channels on CNS brain networks as a new target in pain development.PMC10368246Wank IKreitz SHess ALudwig AHafele MfalseThe impact of HCN4 channels on CNS brain networks as a new target in pain development.While it is well established that the isoform 2 of the hyperpolarization-activated cyclic nucleotide-gated cation channel (HCN2) plays an important role in the development and maintenance of pain, the role of the closely related HCN4 isoform in the processing of nociceptive signals is not known. HCN4 channels are highly expressed in the thalamus, a region important for stimulus transmission and information processing. We used a brain-specific HCN4-knockout mouse line (HCN4-KO) to explore the role of HCN4 channels in acute nociceptive processing using several behavioral tests as well as a multimodal magnetic resonance imaging (MRI) approach. Functional MRI (fMRI) brain responses were measured during acute peripheral thermal stimulation complemented by resting state (RS) before and after sti2023-01-01T00:00:00Z20232025-04-26T11:36:01.984Z2024-12-04T01:38:01.99ZS-EPMC103682463749680310.3389/fnetp.2023.1090502