{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"submitter":["Gandhi SA"],"funding":["NIDDK NIH HHS","NCI NIH HHS"],"pubmed_abstract":["The mechanisms by which the lipid droplet (LD) membrane is remodeled in concert with the activation of lipolysis incorporate a complex interplay of proteins, phospholipids, and neutral lipids. Model LDs (mLDs) provide an isolated, purified system for testing the mechanisms by which the droplet composition, size, shape, and tension affects triglyceride metabolism. Described here are methods of making and testing mLDs ranging from 0.1 to 40 μm diameter with known composition. Methods are described for imaging mLDs with high-resolution microscopy during buffer exchanges for the measurement of membrane binding, diffusion, and tension via fluorescence correlation spectroscopy (FCS), fluorescence recovery after photobleaching (FRAP), fluorescence lifetime imaging microscopy (FLIM), atomic force "],"journal":["bioRxiv : the preprint server for biology"],"pagination":["2023.07.17.549385"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10370146"],"repository":["biostudies-literature"],"pubmed_title":["Methods for making and observing model lipid droplets."],"pmcid":["PMC10370146"],"funding_grant_id":["R01 DK076629","P30 CA022453","R50 CA251068"],"pubmed_authors":["Sanders MA","Truex K","Alduhailan M","Gandhi SA","Junedi N","Hoffmann PM","Kelly CV","Parveen S","Tripathi R","Granneman JG","Saqallah M"],"additional_accession":[]},"is_claimable":false,"name":"Methods for making and observing model lipid droplets.","description":"The mechanisms by which the lipid droplet (LD) membrane is remodeled in concert with the activation of lipolysis incorporate a complex interplay of proteins, phospholipids, and neutral lipids. Model LDs (mLDs) provide an isolated, purified system for testing the mechanisms by which the droplet composition, size, shape, and tension affects triglyceride metabolism. Described here are methods of making and testing mLDs ranging from 0.1 to 40 μm diameter with known composition. Methods are described for imaging mLDs with high-resolution microscopy during buffer exchanges for the measurement of membrane binding, diffusion, and tension via fluorescence correlation spectroscopy (FCS), fluorescence recovery after photobleaching (FRAP), fluorescence lifetime imaging microscopy (FLIM), atomic force ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jul","modification":"2025-04-05T13:57:36.539Z","creation":"2025-04-05T13:57:36.539Z"},"accession":"S-EPMC10370146","cross_references":{"pubmed":["37503132"],"doi":["10.1101/2023.07.17.549385"]}}