{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Grutzmeier SE"],"funding":["Tømrermester Jørgen Holm og Hustru Elisa f. Hansens Mindelegats","Arvd Nilssons Fond","Dagmar Marshalls Fond","Lægeforeningens Forskningsfond","Danish Cancer Society"],"pagination":["3677"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10377599"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["15(14)"],"pubmed_abstract":["Cancer-associated fibroblasts (CAFs) have been shown to impact the chemosensitivity of patient-derived tumor organoids (PDTOs). However, the published literature comparing PDTO response to clinical outcome does not include CAFs in the models. Here, a co-culture model was created using PDTOs and CAFs derived from endoscopic ultrasound-guided fine-needle biopsies (EUS-FNBs) for potential use in drug screening applications. Co-cultures were established, and growth was compared to monocultures using image metrics and a commercially available assay. We were able to establish and expand validated malignant PDTOs from 19.2% of adenocarcinomas from EUS-FNBs. CAFs could be established from 25% of the samples. The viability of PDTOs in the mixed cell co-culture could be isolated using image metrics."],"journal":["Cancers"],"pubmed_title":["Validation of a Novel EUS-FNB-Derived Organoid Co-Culture System for Drug Screening in Patients with Pancreatic Cancer."],"pmcid":["PMC10377599"],"funding_grant_id":["2019-3780/40","F-10215-00-86","R322-A17350","F-19215-00-65.","21081"],"pubmed_authors":["Grossjohann H","Chiranth D","Klausen P","Grutzmeier SE","Kovacevic B","Hasselby JP","Karstensen JG","Rift CV","Hogdall E","Holmstrom MO","Melchior LC","Vilmann P","Hansen CP","Brink L","Toxværd A","Hassan H"],"additional_accession":[]},"is_claimable":false,"name":"Validation of a Novel EUS-FNB-Derived Organoid Co-Culture System for Drug Screening in Patients with Pancreatic Cancer.","description":"Cancer-associated fibroblasts (CAFs) have been shown to impact the chemosensitivity of patient-derived tumor organoids (PDTOs). However, the published literature comparing PDTO response to clinical outcome does not include CAFs in the models. Here, a co-culture model was created using PDTOs and CAFs derived from endoscopic ultrasound-guided fine-needle biopsies (EUS-FNBs) for potential use in drug screening applications. Co-cultures were established, and growth was compared to monocultures using image metrics and a commercially available assay. We were able to establish and expand validated malignant PDTOs from 19.2% of adenocarcinomas from EUS-FNBs. CAFs could be established from 25% of the samples. The viability of PDTOs in the mixed cell co-culture could be isolated using image metrics.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jul","modification":"2025-04-05T00:23:09.857Z","creation":"2025-04-05T00:23:09.857Z"},"accession":"S-EPMC10377599","cross_references":{"pubmed":["37509338"],"doi":["10.3390/cancers15143677"]}}