biostudies-literatureGrutzmeier SETømrermester Jørgen Holm og Hustru Elisa f. Hansens MindelegatsArvd Nilssons FondDagmar Marshalls FondLægeforeningens ForskningsfondDanish Cancer Society3677https://www.ebi.ac.uk/biostudies/studies/S-EPMC10377599biostudies-literatureUnknown15(14)Cancer-associated fibroblasts (CAFs) have been shown to impact the chemosensitivity of patient-derived tumor organoids (PDTOs). However, the published literature comparing PDTO response to clinical outcome does not include CAFs in the models. Here, a co-culture model was created using PDTOs and CAFs derived from endoscopic ultrasound-guided fine-needle biopsies (EUS-FNBs) for potential use in drug screening applications. Co-cultures were established, and growth was compared to monocultures using image metrics and a commercially available assay. We were able to establish and expand validated malignant PDTOs from 19.2% of adenocarcinomas from EUS-FNBs. CAFs could be established from 25% of the samples. The viability of PDTOs in the mixed cell co-culture could be isolated using image metrics.CancersValidation of a Novel EUS-FNB-Derived Organoid Co-Culture System for Drug Screening in Patients with Pancreatic Cancer.PMC103775992019-3780/40F-10215-00-86R322-A17350F-19215-00-65.21081Grossjohann HChiranth DKlausen PGrutzmeier SEKovacevic BHasselby JPKarstensen JGRift CVHogdall EHolmstrom MOMelchior LCVilmann PHansen CPBrink LToxværd AHassan HfalseValidation of a Novel EUS-FNB-Derived Organoid Co-Culture System for Drug Screening in Patients with Pancreatic Cancer.Cancer-associated fibroblasts (CAFs) have been shown to impact the chemosensitivity of patient-derived tumor organoids (PDTOs). However, the published literature comparing PDTO response to clinical outcome does not include CAFs in the models. Here, a co-culture model was created using PDTOs and CAFs derived from endoscopic ultrasound-guided fine-needle biopsies (EUS-FNBs) for potential use in drug screening applications. Co-cultures were established, and growth was compared to monocultures using image metrics and a commercially available assay. We were able to establish and expand validated malignant PDTOs from 19.2% of adenocarcinomas from EUS-FNBs. CAFs could be established from 25% of the samples. The viability of PDTOs in the mixed cell co-culture could be isolated using image metrics.2023-01-01T00:00:00Z2023 Jul2025-04-05T00:23:09.857Z2025-04-05T00:23:09.857ZS-EPMC103775993750933810.3390/cancers15143677