{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Verdile N"],"funding":["European Union’s Horizon 2020 research and innovation program"],"pagination":["1843"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10377977"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(14)"],"pubmed_abstract":["In vitro organotypic cell-based intestinal platforms, able to faithfully recapitulate the complex functions of the organ in vivo, would be a great support to search for more sustainable feed ingredients in aquaculture. We previously demonstrated that proliferation or differentiation of rainbow trout intestinal cell lines is dictated by the culture environment. The aim of the present work was to develop a culture platform that can efficiently promote cell differentiation into mature enterocytes. We compared four options, seeding the RTpiMI cell line derived from the proximal intestine on (1) polyethylene terephthalate (PET) culture inserts ThinCert™ (TC), (2) TC coated with the solubilized basement membrane matrix Matrigel<sup>®</sup> (MM), (3) TC with the rainbow trout fibroblast cell line"],"journal":["Cells"],"pubmed_title":["Distinct Organotypic Platforms Modulate Rainbow Trout (<i>Oncorhynchus mykiss</i>) Intestinal Cell Differentiation In Vitro."],"pmcid":["PMC10377977"],"funding_grant_id":["828835"],"pubmed_authors":["De Noni I","Camin F","Verdile N","Stuknyte M","Pasquariello R","Pavlovic R","Brevini TAL","Gandolfi F"],"additional_accession":[]},"is_claimable":false,"name":"Distinct Organotypic Platforms Modulate Rainbow Trout (<i>Oncorhynchus mykiss</i>) Intestinal Cell Differentiation In Vitro.","description":"In vitro organotypic cell-based intestinal platforms, able to faithfully recapitulate the complex functions of the organ in vivo, would be a great support to search for more sustainable feed ingredients in aquaculture. We previously demonstrated that proliferation or differentiation of rainbow trout intestinal cell lines is dictated by the culture environment. The aim of the present work was to develop a culture platform that can efficiently promote cell differentiation into mature enterocytes. We compared four options, seeding the RTpiMI cell line derived from the proximal intestine on (1) polyethylene terephthalate (PET) culture inserts ThinCert™ (TC), (2) TC coated with the solubilized basement membrane matrix Matrigel<sup>®</sup> (MM), (3) TC with the rainbow trout fibroblast cell line","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Jul","modification":"2026-06-20T03:12:54.12Z","creation":"2025-04-05T00:23:03.232Z"},"accession":"S-EPMC10377977","cross_references":{"pubmed":["37508507"],"doi":["10.3390/cells12141843"]}}