biostudies-literaturePadilla-Benavides TNIH HHS11256https://www.ebi.ac.uk/biostudies/studies/S-EPMC10378909biostudies-literatureUnknown24(14)Mammalian SWI/SNF (mSWI/SNF) complexes are ATP-dependent chromatin remodeling enzymes that are critical for normal cellular functions. mSWI/SNF enzymes are classified into three sub-families based on the presence of specific subunit proteins. The sub-families are Brm- or Brg1-associated factor (BAF), ncBAF (non-canonical BAF), and polybromo-associated BAF (PBAF). The biological roles for the different enzyme sub-families are poorly described. We knocked down the expression of genes encoding unique subunit proteins for each sub-family, <i>Baf250A</i>, <i>Brd9</i>, and <i>Baf180</i>, which mark the BAF, ncBAF, and PBAF sub-families, respectively, and examined the requirement for each in myoblast differentiation. We found that Baf250A and the BAF complex were required to drive lineage-specifiInternational journal of molecular sciencesDifferential Contributions of mSWI/SNF Chromatin Remodeler Sub-Families to Myoblast Differentiation.PMC10378909R35GM136393R01AR077578Padilla-Benavides TOlea-Flores MZhang KSyed SAZuniga-Eulogio MDSharma TNavarro-Tito NWitwicka HImbalzano ANfalseDifferential Contributions of mSWI/SNF Chromatin Remodeler Sub-Families to Myoblast Differentiation.Mammalian SWI/SNF (mSWI/SNF) complexes are ATP-dependent chromatin remodeling enzymes that are critical for normal cellular functions. mSWI/SNF enzymes are classified into three sub-families based on the presence of specific subunit proteins. The sub-families are Brm- or Brg1-associated factor (BAF), ncBAF (non-canonical BAF), and polybromo-associated BAF (PBAF). The biological roles for the different enzyme sub-families are poorly described. We knocked down the expression of genes encoding unique subunit proteins for each sub-family, <i>Baf250A</i>, <i>Brd9</i>, and <i>Baf180</i>, which mark the BAF, ncBAF, and PBAF sub-families, respectively, and examined the requirement for each in myoblast differentiation. We found that Baf250A and the BAF complex were required to drive lineage-specifi2023-01-01T00:00:00Z2023 Jul2025-04-05T14:50:54.227Z2025-04-05T14:50:54.227ZS-EPMC103789093751101610.3390/ijms241411256