biostudies-literatureZhu WModern Agroindustry Technology Research SystemNational Natural Science Foundation of China11573https://www.ebi.ac.uk/biostudies/studies/S-EPMC10380378biostudies-literatureUnknown24(14)DNA methylation is an important epigenetic modification that has been shown to be associated with responses to non-biological stressors. However, there is currently no research on DNA methylation in response to environmental signals in shrimp. In this study, we conducted a comprehensive comparative analysis of DNA methylation profiles and differentially expressed genes between two strains of <i>Litopenaeus vannamei</i> with significantly different cold tolerance through whole genome bisulfite sequencing (WGBS) and transcriptome sequencing. Between Lv-C and Lv-T (constant temperature of 28 °C and low temperatures of 18 °C and 10 °C) under cytosine-guanine (CG) environments, 39,100 differentially methylated regions (DMRs) were identified, corresponding to 9302 DMR-related genes (DMRGs). The International journal of molecular sciencesIntegrated Analysis of DNA Methylome and Transcriptome Reveals Epigenetic Regulation of Cold Tolerance in <i>Litopenaeus vannamei</i>.PMC103803783207299632260916CARS-48Liu QLi QZhu WWang HChen XYang CPeng MZhang BFeng PZeng DZhao YChen TfalseIntegrated Analysis of DNA Methylome and Transcriptome Reveals Epigenetic Regulation of Cold Tolerance in <i>Litopenaeus vannamei</i>.DNA methylation is an important epigenetic modification that has been shown to be associated with responses to non-biological stressors. However, there is currently no research on DNA methylation in response to environmental signals in shrimp. In this study, we conducted a comprehensive comparative analysis of DNA methylation profiles and differentially expressed genes between two strains of <i>Litopenaeus vannamei</i> with significantly different cold tolerance through whole genome bisulfite sequencing (WGBS) and transcriptome sequencing. Between Lv-C and Lv-T (constant temperature of 28 °C and low temperatures of 18 °C and 10 °C) under cytosine-guanine (CG) environments, 39,100 differentially methylated regions (DMRs) were identified, corresponding to 9302 DMR-related genes (DMRGs). The 2023-01-01T00:00:00Z2023 Jul2025-04-20T03:16:00.596Z2025-04-20T03:16:00.596ZS-EPMC103803783751133210.3390/ijms241411573