biostudies-literatureLecona AMConsejo Nacional de Ciencia y TecnologíaNational Autonomous University of MexicoCONACyTDGAPA/PAPIIT-UNAM11720https://www.ebi.ac.uk/biostudies/studies/S-EPMC10380779biostudies-literatureUnknown24(14)Cysteine-rich receptor-like kinases (CRKs) are a type of receptor-like kinases (RLKs) that are important for pathogen resistance, extracellular reactive oxygen species (ROS) signaling, and programmed cell death in plants. In a previous study, we identified 46 CRK family members in the <i>Phaseolus vulgaris</i> genome and found that <i>CRK12</i> was highly upregulated under root nodule symbiotic conditions. To better understand the role of <i>CRK12</i> in the <i>Phaseolus</i>-<i>Rhizobia</i> symbiotic interaction, we functionally characterized this gene by overexpressing (<i>CRK12</i>-OE) and silencing (<i>CRK12</i>-RNAi) it in a <i>P. vulgaris</i> hairy root system. We found that the constitutive expression of <i>CRK12</i> led to an increase in root hair length and the expression of root hInternational journal of molecular sciences<i>CRK12</i>: A Key Player in Regulating the <i>Phaseolus vulgaris</i>-<i>Rhizobium tropici</i> Symbiotic Interaction.PMC10380779CF-MI-20191017134234199-316538IN216321 and IN213221IN216321IN213221Lecona AMNanjareddy KVera-Nunez JAPiazza VLara MBlanco LArthikala MKfalse<i>CRK12</i>: A Key Player in Regulating the <i>Phaseolus vulgaris</i>-<i>Rhizobium tropici</i> Symbiotic Interaction.Cysteine-rich receptor-like kinases (CRKs) are a type of receptor-like kinases (RLKs) that are important for pathogen resistance, extracellular reactive oxygen species (ROS) signaling, and programmed cell death in plants. In a previous study, we identified 46 CRK family members in the <i>Phaseolus vulgaris</i> genome and found that <i>CRK12</i> was highly upregulated under root nodule symbiotic conditions. To better understand the role of <i>CRK12</i> in the <i>Phaseolus</i>-<i>Rhizobia</i> symbiotic interaction, we functionally characterized this gene by overexpressing (<i>CRK12</i>-OE) and silencing (<i>CRK12</i>-RNAi) it in a <i>P. vulgaris</i> hairy root system. We found that the constitutive expression of <i>CRK12</i> led to an increase in root hair length and the expression of root h2023-01-01T00:00:00Z2023 Jul2025-07-04T03:06:01.15Z2025-02-19T03:56:13.994ZS-EPMC103807793751147910.3390/ijms241411720