biostudies-literatureSilva AJDFoundation for the Support of Science and Technology of PernambucoNational Council for Scientific and Technological Development1898https://www.ebi.ac.uk/biostudies/studies/S-EPMC10385271biostudies-literatureUnknown15(7)Improving antigen presentation is crucial for the success of immunization strategies. Yeasts are classically used as biofactories to produce recombinant proteins and are efficient vehicles for antigen delivery, in addition to their adjuvant properties. Despite the absence of epidemic outbreaks, several vaccine approaches continue to be developed for Zika virus infection. The development of these prophylactic strategies is fundamental given the severity of clinical manifestations, mainly due to viral neurotropism. The present study aimed to evaluate in vivo the immune response induced by <i>P. pastoris</i> recombinant strains displaying epitopes of the envelope (ENV) and NS1 ZIKV proteins. Intramuscular immunization with heat-attenuated yeast enhanced the secretion of IL-6, TNF-α, and IFN-γPharmaceuticsWhole Yeast Vaccine Displaying ZIKV B and T Cell Epitopes Induces Cellular Immune Responses in the Murine Model.PMC10385271APQ-1482-2.02/2288887.507421/ 2020-00440315/2022-0Pena LJSilva AJDLeal LRSde Freitas ACda Paz Leoncio Alves Sde Melo CMLde Jesus ALSde Macedo LSde Sousa GFda Silva Barros BRfalseWhole Yeast Vaccine Displaying ZIKV B and T Cell Epitopes Induces Cellular Immune Responses in the Murine Model.Improving antigen presentation is crucial for the success of immunization strategies. Yeasts are classically used as biofactories to produce recombinant proteins and are efficient vehicles for antigen delivery, in addition to their adjuvant properties. Despite the absence of epidemic outbreaks, several vaccine approaches continue to be developed for Zika virus infection. The development of these prophylactic strategies is fundamental given the severity of clinical manifestations, mainly due to viral neurotropism. The present study aimed to evaluate in vivo the immune response induced by <i>P. pastoris</i> recombinant strains displaying epitopes of the envelope (ENV) and NS1 ZIKV proteins. Intramuscular immunization with heat-attenuated yeast enhanced the secretion of IL-6, TNF-α, and IFN-γ2023-01-01T00:00:00Z2023 Jul2025-04-05T12:35:20.776Z2025-04-05T12:35:20.776ZS-EPMC103852713751408410.3390/pharmaceutics15071898