biostudies-literatureYu QSichuan Provincial Key Research Project for Forages BreedingKey R & D project of Sichuan Science and Technology ProgramNational Natural Science Foundation of ChinaNational Modern Forages Industry Technology SystemEmergency Sci. & Tech Project from National Forestry and Grassland Administration ('Breeding and Cultivation of Fine Herbage Varieties')Sichuan Provincial Regional Innovation Cooperation Project2719https://www.ebi.ac.uk/biostudies/studies/S-EPMC10385362biostudies-literatureUnknown12(14)Drought is one of the most significant limiting factors affecting plant growth and development on the Qinghai-Tibet Plateau (QTP). Mining the drought-tolerant genes of the endemic perennial grass of the QTP, Siberian wildrye (<i>Elymus sibiricus</i>), is of great significance to creating new drought-resistant varieties which can be used in the development of grassland livestock and restoring natural grassland projects in the QTP. To investigate the transcriptomic responsiveness of <i>E. sibiricus</i> to drought stress, PEG-induced short- and long-term drought stress was applied to two Siberian wildrye genotypes (drought-tolerant and drought-sensitive accessions), followed by third- and second-generation transcriptome sequencing analysis. A total of 40,708 isoforms were detected, of which 1Plants (Basel, Switzerland)Integrating Full-Length Transcriptome and RNA Sequencing of Siberian Wildrye (<i>Elymus sibiricus</i>) to Reveal Molecular Mechanisms in Response to Drought Stress.PMC103853622021YFYZ0013-22022YFQ0076CARS-342023YFSY00123177266120220102Xiong YYan LBai SLei XYu QDong ZMa XShu XSu XZhao JfalseIntegrating Full-Length Transcriptome and RNA Sequencing of Siberian Wildrye (<i>Elymus sibiricus</i>) to Reveal Molecular Mechanisms in Response to Drought Stress.Drought is one of the most significant limiting factors affecting plant growth and development on the Qinghai-Tibet Plateau (QTP). Mining the drought-tolerant genes of the endemic perennial grass of the QTP, Siberian wildrye (<i>Elymus sibiricus</i>), is of great significance to creating new drought-resistant varieties which can be used in the development of grassland livestock and restoring natural grassland projects in the QTP. To investigate the transcriptomic responsiveness of <i>E. sibiricus</i> to drought stress, PEG-induced short- and long-term drought stress was applied to two Siberian wildrye genotypes (drought-tolerant and drought-sensitive accessions), followed by third- and second-generation transcriptome sequencing analysis. A total of 40,708 isoforms were detected, of which 12023-01-01T00:00:00Z2023 Jul2025-04-22T13:33:41.237Z2025-04-06T00:40:20.053ZS-EPMC103853623751433310.3390/plants12142719