{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Zhang Q"],"funding":["National Natural Science Foundation of China"],"pagination":["3127-3151"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10396372"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["35(8)"],"pubmed_abstract":["Endomembrane remodeling to form a viral replication complex (VRC) is crucial for a virus to establish infection in a host. Although the composition and function of VRCs have been intensively studied, host factors involved in the assembly of VRCs for plant RNA viruses have not been fully explored. TurboID-based proximity labeling (PL) has emerged as a robust tool for probing molecular interactions in planta. However, few studies have employed the TurboID-based PL technique for investigating plant virus replication. Here, we used Beet black scorch virus (BBSV), an endoplasmic reticulum (ER)-replicating virus, as a model and systematically investigated the composition of BBSV VRCs in Nicotiana benthamiana by fusing the TurboID enzyme to viral replication protein p23. Among the 185 identified "],"journal":["The Plant cell"],"pubmed_title":["RETICULON-LIKE PROTEIN B2 is a proviral factor co-opted for the biogenesis of viral replication organelles in plants."],"pmcid":["PMC10396372"],"funding_grant_id":["31872637","32122070"],"pubmed_authors":["Wen Z","Li Z","Wang R","Li D","Zhang Y","Zhang X","She J","Gao Z","Wang X","Su Z","Zhang Q","Zhao X"],"additional_accession":[]},"is_claimable":false,"name":"RETICULON-LIKE PROTEIN B2 is a proviral factor co-opted for the biogenesis of viral replication organelles in plants.","description":"Endomembrane remodeling to form a viral replication complex (VRC) is crucial for a virus to establish infection in a host. Although the composition and function of VRCs have been intensively studied, host factors involved in the assembly of VRCs for plant RNA viruses have not been fully explored. TurboID-based proximity labeling (PL) has emerged as a robust tool for probing molecular interactions in planta. However, few studies have employed the TurboID-based PL technique for investigating plant virus replication. Here, we used Beet black scorch virus (BBSV), an endoplasmic reticulum (ER)-replicating virus, as a model and systematically investigated the composition of BBSV VRCs in Nicotiana benthamiana by fusing the TurboID enzyme to viral replication protein p23. Among the 185 identified ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Aug","modification":"2025-07-04T03:05:51.899Z","creation":"2025-02-19T01:58:15.261Z"},"accession":"S-EPMC10396372","cross_references":{"pubmed":["37216674"],"doi":["10.1093/plcell/koad146"]}}