{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Yang ASP"],"funding":["European Research Council","Dutch Research Council (NWO)","The Netherlands Organisation for Health Research and Development Off-Road grant","ZonMw"],"pagination":["4631"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10397232"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["14(1)"],"pubmed_abstract":["Plasmodium falciparum (Pf) parasite development in liver represents the initial step of the life-cycle in the human host after a Pf-infected mosquito bite. While an attractive stage for life-cycle interruption, understanding of parasite-hepatocyte interaction is inadequate due to limitations of existing in vitro models. We explore the suitability of hepatocyte organoids (HepOrgs) for Pf-development and show that these cells permitted parasite invasion, differentiation and maturation of different Pf strains. Single-cell messenger RNA sequencing (scRNAseq) of Pf-infected HepOrg cells has identified 80 Pf-transcripts upregulated on day 5 post-infection. Transcriptional profile changes are found involving distinct metabolic pathways in hepatocytes with Scavenger Receptor B1 (SR-B1) transcripts highly upregulated. A novel functional involvement in schizont maturation is confirmed in fresh primary hepatocytes. Thus, HepOrgs provide a strong foundation for a versatile in vitro model for Pf liver-stages accommodating basic biological studies and accelerated clinical development of novel tools for malaria control."],"journal":["Nature communications"],"pubmed_title":["Development of Plasmodium falciparum liver-stages in hepatocytes derived from human fetal liver organoid cultures."],"pmcid":["PMC10397232"],"funding_grant_id":["04510012010050","VI.Veni.212.134","VI.Veni.192.171","016.Veni.171.015","016.166.140","101042738"],"pubmed_authors":["Kretzschmar K","Boonekamp KE","Yang ASP","Clevers H","Hendriks D","Sauerwein RW","Dutta D","van Waardenburg Y","Chuva de Sousa Lopes SM","Hu H","Puschhof J","van Gemert GJ","de Wilt JHW","Bousema T"],"additional_accession":[]},"is_claimable":false,"name":"Development of Plasmodium falciparum liver-stages in hepatocytes derived from human fetal liver organoid cultures.","description":"Plasmodium falciparum (Pf) parasite development in liver represents the initial step of the life-cycle in the human host after a Pf-infected mosquito bite. While an attractive stage for life-cycle interruption, understanding of parasite-hepatocyte interaction is inadequate due to limitations of existing in vitro models. We explore the suitability of hepatocyte organoids (HepOrgs) for Pf-development and show that these cells permitted parasite invasion, differentiation and maturation of different Pf strains. Single-cell messenger RNA sequencing (scRNAseq) of Pf-infected HepOrg cells has identified 80 Pf-transcripts upregulated on day 5 post-infection. Transcriptional profile changes are found involving distinct metabolic pathways in hepatocytes with Scavenger Receptor B1 (SR-B1) transcripts highly upregulated. A novel functional involvement in schizont maturation is confirmed in fresh primary hepatocytes. Thus, HepOrgs provide a strong foundation for a versatile in vitro model for Pf liver-stages accommodating basic biological studies and accelerated clinical development of novel tools for malaria control.","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Aug","modification":"2025-04-18T17:01:13.57Z","creation":"2025-04-07T04:31:15.244Z"},"accession":"S-EPMC10397232","cross_references":{"pubmed":["37532704"],"doi":["10.1038/s41467-023-40298-7"]}}