biostudies-literatureSiegle JHNIBIB NIH HHS86-92https://www.ebi.ac.uk/biostudies/studies/S-EPMC10399640biostudies-literatureUnknown592(7852)The anatomy of the mammalian visual system, from the retina to the neocortex, is organized hierarchically¹. However, direct observation of cellular-level functional interactions across this hierarchy is lacking due to the challenge of simultaneously recording activity across numerous regions. Here we describe a large, open dataset-part of the Allen Brain Observatory²-that surveys spiking from tens of thousands of units in six cortical and two thalamic regions in the brains of mice responding to a battery of visual stimuli. Using cross-correlation analysis, we reveal that the organization of inter-area functional connectivity during visual stimulation mirrors the anatomical hierarchy from the Allen Mouse Brain Connectivity Atlas³. We find that four classicalNatureSurvey of spiking in the mouse visual system reveals functional hierarchy.PMC10399640R01 EB026908Sutton BThompson CCox TCNaylor SCasal LIyer Rde Vries SEJWilliams DKoch CAhmed RArkhipov ABrown DHancock NDurand SLiang ENayan CCaldejon SGalbraith JWilliford AWakeman WLambert SPhillips JWNgo KPerkins JLee JHPachitariu MMihalas SGelfand ECMollenkopf TLeon AMace KReding MLecoq JOllerenshaw DHu BStoecklin MMelchior JKiggins JLi YYoung RNicovich PRWhitesell JDLedochowitsch PGarrett MZeng HBilleh YNDenman DJJohnson KLong FRonellenfitch KChoi HLuviano JASwapp JMillman DCho AFeng DCain NSullivan DHoward RHytnen RKato IFarrell CTurner KEsposito LGale SJia XGraddis NNorth KSlaughterbeck CJessett ENg LSeid SGroblewski PABennett CChvilicek MHeller GNguyen THarris JASiegle JHRamirez TKBuice MAReid RCBernard ADai KDietzman ROlsen SROliver MRobertson MOcker GKReid DfalseSurvey of spiking in the mouse visual system reveals functional hierarchy.The anatomy of the mammalian visual system, from the retina to the neocortex, is organized hierarchically¹. However, direct observation of cellular-level functional interactions across this hierarchy is lacking due to the challenge of simultaneously recording activity across numerous regions. Here we describe a large, open dataset-part of the Allen Brain Observatory²-that surveys spiking from tens of thousands of units in six cortical and two thalamic regions in the brains of mice responding to a battery of visual stimuli. Using cross-correlation analysis, we reveal that the organization of inter-area functional connectivity during visual stimulation mirrors the anatomical hierarchy from the Allen Mouse Brain Connectivity Atlas³. We find that four classical2021-01-01T00:00:00Z2021 Apr2025-04-22T13:40:02.461Z2025-04-06T00:43:16.188ZS-EPMC103996403347321610.1038/s41586-020-03171-x