{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Fatemi E"],"funding":["The German Federal Ministry of Education and Research","Christian-Albrechts-Universität zu Kiel"],"pagination":["12602"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10400682"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["Root lesion nematodes (RLN) of the genus Pratylenchus are causing significant damage in cereal production worldwide. Due to climate change and without efficient and environment-friendly treatments, the damages through RLNs are predicted to increase. Microscopic assessments of RLNs in the field and the greenhouses are time-consuming and laborious. As a result, cereal breeders have mostly ignored this pest. We present a method measuring RLN in infected cereal roots using a standardized PCR approach. Publicly available Pratylenchus neglectus primer combinations were evaluated. An optimal primer combination for RT-qPCR assay was identified to detect and quantify P. neglectus within infected cereal roots. Using the RT-qPCR detection assay, P. neglectus could be clearly distinguished from other "],"journal":["Scientific reports"],"pubmed_title":["DNA-based assessment of root lesion nematode infections in cereal roots."],"pmcid":["PMC10400682"],"funding_grant_id":["031B0186A"],"pubmed_authors":["Melzer S","Jung C","Fatemi E"],"additional_accession":[]},"is_claimable":false,"name":"DNA-based assessment of root lesion nematode infections in cereal roots.","description":"Root lesion nematodes (RLN) of the genus Pratylenchus are causing significant damage in cereal production worldwide. Due to climate change and without efficient and environment-friendly treatments, the damages through RLNs are predicted to increase. Microscopic assessments of RLNs in the field and the greenhouses are time-consuming and laborious. As a result, cereal breeders have mostly ignored this pest. We present a method measuring RLN in infected cereal roots using a standardized PCR approach. Publicly available Pratylenchus neglectus primer combinations were evaluated. An optimal primer combination for RT-qPCR assay was identified to detect and quantify P. neglectus within infected cereal roots. Using the RT-qPCR detection assay, P. neglectus could be clearly distinguished from other ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Aug","modification":"2026-05-28T21:37:29.666Z","creation":"2024-11-08T10:01:34.644Z"},"accession":"S-EPMC10400682","cross_references":{"pubmed":["37537261"],"doi":["10.1038/s41598-023-39559-8"]}}