{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["62(30)"],"submitter":["Gallifuoco A"],"pubmed_abstract":["This paper explores the potentialities of hydrochar in protein separation and enzyme immobilization for non-energy biorefinery applications of hydrothermal carbonization. An innovative experimental procedure monitors soluble protein-hydrochar interactions and enzymatic reactions in a continuously stirred tank reactor. The hydrochar comes from hydrothermal carbonization of silver fir (200 °C, 30 min, 1/7 solid/water ratio) and standard activation (KOH, oven, 600 °C). Bovine serum albumin, a non-active, globular protein, was adsorbed at ≤3300 mg/g. Sip's isotherms fitted data well (<i>R</i><sup>2</sup> = 0.99999). The immobilization used a commercial β-glucosidase, which catalyzes the hydrolysis of cellobiose to glucose, a bottleneck of the cellulose to fermentable sugar bioconversion networ"],"journal":["Industrial & engineering chemistry research"],"pagination":["11805-11813"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10401700"],"repository":["biostudies-literature"],"pubmed_title":["An Exploratory Study of Hydrochar as a Matrix for Biotechnological Applications."],"pmcid":["PMC10401700"],"pubmed_authors":["Spera A","Papa AA","Taglieri L","Di Carlo A","Gallifuoco A","Passucci M"],"additional_accession":[]},"is_claimable":false,"name":"An Exploratory Study of Hydrochar as a Matrix for Biotechnological Applications.","description":"This paper explores the potentialities of hydrochar in protein separation and enzyme immobilization for non-energy biorefinery applications of hydrothermal carbonization. An innovative experimental procedure monitors soluble protein-hydrochar interactions and enzymatic reactions in a continuously stirred tank reactor. The hydrochar comes from hydrothermal carbonization of silver fir (200 °C, 30 min, 1/7 solid/water ratio) and standard activation (KOH, oven, 600 °C). Bovine serum albumin, a non-active, globular protein, was adsorbed at ≤3300 mg/g. Sip's isotherms fitted data well (<i>R</i><sup>2</sup> = 0.99999). The immobilization used a commercial β-glucosidase, which catalyzes the hydrolysis of cellobiose to glucose, a bottleneck of the cellulose to fermentable sugar bioconversion networ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Aug","modification":"2025-04-20T03:16:56.018Z","creation":"2025-02-19T01:58:08.482Z"},"accession":"S-EPMC10401700","cross_references":{"pubmed":["37546184"],"doi":["10.1021/acs.iecr.3c00765"]}}