biostudies-literatureThai NMHue University under the Core Research ProgramCooperative Research Programme between Institute of Applied Research in Science and Technology, University of Sciences, Hue University and KeFa Science and Technology Co., LtdHue Unversity292https://www.ebi.ac.uk/biostudies/studies/S-EPMC10403485biostudies-literatureUnknown13(9)Laboratory experiments were carried out to identify the chemical composition of <i>Cordyceps militaris</i> and reveal the first evidence of their Alzheimer-related potential. Liquid chromatography-mass spectrometry analysis identified 21 bioactive compounds in the ethanol extract (<b>1-21</b>). High-performance liquid chromatography quantified the content of cordycepin (0.32%). Bioassays revealed the overall anti-Alzheimer potential of the extract against acetylcholinesterase (IC₅₀ = 115.9 ± 11.16 µg mL^-1). Multi-platform computations were utilized to predict the biological inhibitory effects of its phytochemical components against Alzheimer-related protein structures: acetylcholinesterase (PDB-4EY7) and β-amyloid protein (PDB-2LMN). In particular, <b>7</b> is conside3 BiotechIdentification of potential inhibitors against Alzheimer-related proteins in <i>Cordyceps militaris</i> ethanol extract: experimental evidence and computational analyses.PMC10403485DHH2022-01-198NCM.DHH.2020.04Hai NTTNhung NTATriet NTDe Tran VBui TQThai NMPhu NVDat TTHQuy PTPham DTfalseIdentification of potential inhibitors against Alzheimer-related proteins in <i>Cordyceps militaris</i> ethanol extract: experimental evidence and computational analyses.Laboratory experiments were carried out to identify the chemical composition of <i>Cordyceps militaris</i> and reveal the first evidence of their Alzheimer-related potential. Liquid chromatography-mass spectrometry analysis identified 21 bioactive compounds in the ethanol extract (<b>1-21</b>). High-performance liquid chromatography quantified the content of cordycepin (0.32%). Bioassays revealed the overall anti-Alzheimer potential of the extract against acetylcholinesterase (IC₅₀ = 115.9 ± 11.16 µg mL^-1). Multi-platform computations were utilized to predict the biological inhibitory effects of its phytochemical components against Alzheimer-related protein structures: acetylcholinesterase (PDB-4EY7) and β-amyloid protein (PDB-2LMN). In particular, <b>7</b> is conside2023-01-01T00:00:00Z2023 Sep2026-05-04T06:49:44.838Z2025-04-06T13:45:18.71ZS-EPMC104034853754791810.1007/s13205-023-03714-9