biostudies-literatureUnknown97(9)Marcelis QThe in chemico direct peptide reactivity assay (DPRA) is validated to assess protein reactivity of chemical compounds, relating to the molecular initiating event of skin sensitization induction. According to OECD TG 442C, the DPRA is technically applicable to test multi-constituent substances and mixtures of known composition, even though limited experimental data are publicly available. First, we assessed the DPRA's predictive capability for individual substances, but at concentrations other than the recommended 100 mM, i.e., based on the LLNA EC3 concentration (Experiment A). Next, the applicability of the DPRA to test unknown mixtures was assessed (Experiment B). Here, the complexity of unknown mixtures was reduced to mixtures containing either two known skin sensitizers with varying poArchives of toxicology2453-2461https://www.ebi.ac.uk/biostudies/studies/S-EPMC10404183biostudies-literatureApplicability of the DPRA on mixture testing: challenges and opportunities.PMC10404183Desmedt BMarcelis QDeconinck ERogiers VVanhaecke TfalseApplicability of the DPRA on mixture testing: challenges and opportunities.The in chemico direct peptide reactivity assay (DPRA) is validated to assess protein reactivity of chemical compounds, relating to the molecular initiating event of skin sensitization induction. According to OECD TG 442C, the DPRA is technically applicable to test multi-constituent substances and mixtures of known composition, even though limited experimental data are publicly available. First, we assessed the DPRA's predictive capability for individual substances, but at concentrations other than the recommended 100 mM, i.e., based on the LLNA EC3 concentration (Experiment A). Next, the applicability of the DPRA to test unknown mixtures was assessed (Experiment B). Here, the complexity of unknown mixtures was reduced to mixtures containing either two known skin sensitizers with varying po2023-01-01T00:00:00Z2023 Sep2025-04-22T00:55:04.102Z2024-11-08T14:20:42.51ZS-EPMC104041833741488410.1007/s00204-023-03551-y