biostudies-literatureJaber MJustus-Liebig-Universität GießenDeutsche Forschungsgemeinschaft5193-5204https://www.ebi.ac.uk/biostudies/studies/S-EPMC10404207biostudies-literatureUnknown415(21)The Arxula yeast bisphenol screen (A-YBS) utilizes the bioluminescent Arxula adeninivorans yeast-based reporter cells for tailored analysis of bisphenols, one of the major endocrine-disrupting compound groups. For the first time, this bioreporter has been applied on the high-performance thin-layer chromatography (HPTLC) adsorbent surface to develop a respective planar bioluminescence bioassay (pA-YBS). The goal was to combine the advantages of HPTLC with a more selective bioassay detection for bisphenols. The performance of this pA-YBS bioluminescence bioassay was demonstrated by calculating the half-maximal effective concentration (EC₅₀) of bisphenols compared to references. The EC₅₀ ranged from 267 pg/band for bisphenol Z and 322 pg/band for bisphenol A (BPA) to > 1Analytical and bioanalytical chemistryScreening bisphenols in complex samples via a planar Arxula adeninivorans bioluminescence bioassay.PMC10404207INST 162/536-1 FUGGINST 162/471-1 FUGGJaber MOberle MJahne MMorlock GEfalseScreening bisphenols in complex samples via a planar Arxula adeninivorans bioluminescence bioassay.The Arxula yeast bisphenol screen (A-YBS) utilizes the bioluminescent Arxula adeninivorans yeast-based reporter cells for tailored analysis of bisphenols, one of the major endocrine-disrupting compound groups. For the first time, this bioreporter has been applied on the high-performance thin-layer chromatography (HPTLC) adsorbent surface to develop a respective planar bioluminescence bioassay (pA-YBS). The goal was to combine the advantages of HPTLC with a more selective bioassay detection for bisphenols. The performance of this pA-YBS bioluminescence bioassay was demonstrated by calculating the half-maximal effective concentration (EC₅₀) of bisphenols compared to references. The EC₅₀ ranged from 267 pg/band for bisphenol Z and 322 pg/band for bisphenol A (BPA) to > 12023-01-01T00:00:00Z2023 Sep2026-06-05T07:04:50.373Z2025-04-07T04:30:59.292ZS-EPMC104042073745878210.1007/s00216-023-04820-6