biostudies-literatureYou YNational Natural Science Foundation of China12722https://www.ebi.ac.uk/biostudies/studies/S-EPMC10404263biostudies-literatureUnknown13(1)Approximately 20% of aged captive giant pandas (Ailuropoda melanoleuca) have cataracts that impair their quality of life. To identify potential biomarkers of cataract formation, we carried out a quantitative proteomics analysis of 10 giant pandas to find proteins differing in abundance between healthy and cataract-bearing animals. We identified almost 150 proteins exceeding our threshold for differential abundance, most of which were associated with GO categories related to extracellular localization. The most significant differential abundance was associated with components of the proteasome and other proteins with a role in proteolysis or its regulation, most of which were depleted in pandas with cataracts. Other modulated proteins included components of the extracellular matrix or cytosScientific reportsComparative proteomics in captive giant pandas to identify proteins involved in age-related cataract formation.PMC10404263NSFC 31872257Yin YYou YBai CLi JLu YHu XDing NMa TWang LLiu XLiu YZhan TXia MXiu YJia TZhang YHao FZheng CWang WChen YNiu LZhang CPu TZhou JfalseComparative proteomics in captive giant pandas to identify proteins involved in age-related cataract formation.Approximately 20% of aged captive giant pandas (Ailuropoda melanoleuca) have cataracts that impair their quality of life. To identify potential biomarkers of cataract formation, we carried out a quantitative proteomics analysis of 10 giant pandas to find proteins differing in abundance between healthy and cataract-bearing animals. We identified almost 150 proteins exceeding our threshold for differential abundance, most of which were associated with GO categories related to extracellular localization. The most significant differential abundance was associated with components of the proteasome and other proteins with a role in proteolysis or its regulation, most of which were depleted in pandas with cataracts. Other modulated proteins included components of the extracellular matrix or cytos2023-01-01T00:00:00Z2023 Aug2026-04-12T23:16:02.432Z2025-02-19T00:38:37.563ZS-EPMC104042633754364410.1038/s41598-023-40003-0