biostudies-literatureKim JHNational Institute of Horticultural and Herbal Scienses, RDA2242704https://www.ebi.ac.uk/biostudies/studies/S-EPMC10405751biostudies-literatureUnknown38(1)Coronavirus disease 2019 (COVID-19) is an infectious disease caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). 3CLpro is a key enzyme in coronavirus proliferation and a treatment target for COVID-19. In vitro and in silico, compounds 1-3 from Glycyrrhiza uralensis had inhibitory activity and binding affinity for 3CLpro. These compounds decreased HCoV-OC43 cytotoxicity in RD cells. Moreover, they inhibited viral growth by reducing the amounts of the necessary proteins (M, N, and RDRP). Therefore, compounds 1-3 are inhibitors of 3CLpro and HCoV-OC43 proliferation.Journal of enzyme inhibition and medicinal chemistryInhibition by components of Glycyrrhiza uralensis of 3CLpro and HCoV-OC43 proliferation.PMC10405751PJ016777032023Huh YCPark WTKim TIPark JPark YIMoon YHKim JHKang MHCho CWKang JSHur MfalseInhibition by components of Glycyrrhiza uralensis of 3CLpro and HCoV-OC43 proliferation.Coronavirus disease 2019 (COVID-19) is an infectious disease caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2). 3CLpro is a key enzyme in coronavirus proliferation and a treatment target for COVID-19. In vitro and in silico, compounds 1-3 from Glycyrrhiza uralensis had inhibitory activity and binding affinity for 3CLpro. These compounds decreased HCoV-OC43 cytotoxicity in RD cells. Moreover, they inhibited viral growth by reducing the amounts of the necessary proteins (M, N, and RDRP). Therefore, compounds 1-3 are inhibitors of 3CLpro and HCoV-OC43 proliferation.2023-01-01T00:00:00Z2023 Dec2026-05-29T10:23:26.611Z2025-04-04T20:01:01.409ZS-EPMC104057513753788110.1080/14756366.2023.2242704