biostudies-literatureYu ZHunan Provincial Natural Science Foundation of ChinaTechnology Innovation Project of Yihua Lifestyle Technology Co., Ltd.National Natural Science Foundation of ChinaHunan Provincial Forestry Technological Innovation Funds3269https://www.ebi.ac.uk/biostudies/studies/S-EPMC10422229biostudies-literatureUnknown15(15)The autofluorescence phenomenon is an inherent characteristic of lignified cells. However, in the case of <i>Lophira alata</i> (<i>L. alata</i>), the autofluorescence is nearly imperceptible during occasional fluorescence observations. The aim of this study is to investigate the mechanism behind the quenching of lignin's autofluorescence in <i>L. alata</i> by conducting associated experiments. Notably, the autofluorescence image of <i>L. alata</i> observed using optical microscopy appears to be quite indistinct. Abundant extractives are found in the longitudinal parenchyma, fibers, and vessels of <i>L. alata</i>. Remarkably, when subjected to a benzene-alcohol extraction treatment, the autofluorescence of <i>L. alata</i> becomes progressively enhanced under a fluorescence microscope. AdditPolymersImproving the Autofluorescence of <i>Lophira alata</i> Woody Cells via the Removal of Extractives.PMC1042222932271791YH-JS-JSKF-201708006XLK202107-3321717092020JJ2058Liu YHu JLi TWang XAXu DChang SHan JYu ZHuang QLiu GfalseImproving the Autofluorescence of <i>Lophira alata</i> Woody Cells via the Removal of Extractives.The autofluorescence phenomenon is an inherent characteristic of lignified cells. However, in the case of <i>Lophira alata</i> (<i>L. alata</i>), the autofluorescence is nearly imperceptible during occasional fluorescence observations. The aim of this study is to investigate the mechanism behind the quenching of lignin's autofluorescence in <i>L. alata</i> by conducting associated experiments. Notably, the autofluorescence image of <i>L. alata</i> observed using optical microscopy appears to be quite indistinct. Abundant extractives are found in the longitudinal parenchyma, fibers, and vessels of <i>L. alata</i>. Remarkably, when subjected to a benzene-alcohol extraction treatment, the autofluorescence of <i>L. alata</i> becomes progressively enhanced under a fluorescence microscope. Addit2023-01-01T00:00:00Z2023 Aug2025-04-04T10:09:37.536Z2025-02-19T00:26:41.741ZS-EPMC104222293757116310.3390/polym15153269