biostudies-literatureUnknown30Junger SHighlights • Automated LC-MS/MS demonstrated excellent analytical performance.• Automated LC-MS/MS is suitable for hospital and commercial medical laboratories.• Staff without technical expertise in LC-MS/MS can easily operate the automated LC-MS/MS. <h4>Background</h4> Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a sensitive method with high specificity. However, its routine use in the clinical laboratory is hampered by its high complexity and lack of automation. Studies demonstrate excellent analytical performance using the first fully automated LC-MS/MS for 25-hydroxy vitamin D and immunosuppressant drugs (ISD) in hospital routine laboratories. <h4>Objectives</h4> Our objectives were (1) to verify the suitability of an automated LC-MS/MS in a commercial laboratory, whichJournal of mass spectrometry and advances in the clinical lab1-9https://www.ebi.ac.uk/biostudies/studies/S-EPMC10423925biostudies-literatureAutomated LC-MS/MS: Ready for the clinical routine Laboratory?PMC10423925Shipkova MSchoberl CPeter AJunger SBraitmaier HWieland EDanzl GHoene MLehmann RfalseAutomated LC-MS/MS: Ready for the clinical routine Laboratory?Highlights • Automated LC-MS/MS demonstrated excellent analytical performance.• Automated LC-MS/MS is suitable for hospital and commercial medical laboratories.• Staff without technical expertise in LC-MS/MS can easily operate the automated LC-MS/MS. <h4>Background</h4> Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is a sensitive method with high specificity. However, its routine use in the clinical laboratory is hampered by its high complexity and lack of automation. Studies demonstrate excellent analytical performance using the first fully automated LC-MS/MS for 25-hydroxy vitamin D and immunosuppressant drugs (ISD) in hospital routine laboratories. <h4>Objectives</h4> Our objectives were (1) to verify the suitability of an automated LC-MS/MS in a commercial laboratory, which2023-01-01T00:00:00Z2023 Aug2025-04-19T00:57:01.457Z2025-04-07T11:56:36.798ZS-EPMC10423925