{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Chen C"],"funding":["the Science Technology Commission of Shanghai Municipality","the National Natural Science Foundation of China"],"pagination":["698-710"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10423964"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["24(8)"],"pubmed_abstract":["To explore the role of forkhead box protein O1 (FOXO1) in the progression of glioblastoma multiforme (GBM) and related drug resistance, we deciphered the roles of FOXO1 and miR-506 in proliferation, apoptosis, migration, invasion, autophagy, and temozolomide (TMZ) sensitivity in the U251 cell line using in vitro and in vivo experiments. Cell viability was tested by a cell counting kit-8 (CCK8) kit; migration and invasion were checked by the scratching assay; apoptosis was evaluated by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining and flow cytometry. The construction of plasmids and dual-luciferase reporter experiment were carried out to find the interaction site between FOXO1 and miR-506. Immunohistochemistry was done to check the protein level in tumors aft"],"journal":["Journal of Zhejiang University. Science. B"],"pubmed_title":["FOXO1-miR-506 axis promotes chemosensitivity to temozolomide and suppresses invasiveness in glioblastoma through a feedback loop of FOXO1/miR-506/ETS1/FOXO1."],"pmcid":["PMC10423964"],"funding_grant_id":["81402076, 81872072 and 82073274","20S11900700"],"pubmed_authors":["Liu Y","Chen J","Zhang X","Wang H","Chen C","Shi Y"],"additional_accession":[]},"is_claimable":false,"name":"FOXO1-miR-506 axis promotes chemosensitivity to temozolomide and suppresses invasiveness in glioblastoma through a feedback loop of FOXO1/miR-506/ETS1/FOXO1.","description":"To explore the role of forkhead box protein O1 (FOXO1) in the progression of glioblastoma multiforme (GBM) and related drug resistance, we deciphered the roles of FOXO1 and miR-506 in proliferation, apoptosis, migration, invasion, autophagy, and temozolomide (TMZ) sensitivity in the U251 cell line using in vitro and in vivo experiments. Cell viability was tested by a cell counting kit-8 (CCK8) kit; migration and invasion were checked by the scratching assay; apoptosis was evaluated by terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining and flow cytometry. The construction of plasmids and dual-luciferase reporter experiment were carried out to find the interaction site between FOXO1 and miR-506. Immunohistochemistry was done to check the protein level in tumors aft","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Aug","modification":"2025-04-05T15:46:57.886Z","creation":"2025-04-05T15:46:57.886Z"},"accession":"S-EPMC10423964","cross_references":{"pubmed":["37551556"],"doi":["10.1631/jzus.B2200503","10.1631/jzus.b2200503"]}}