biostudies-literatureUnknown18(1)Yang BAlkbh5 is one of the primary demethylases responsible for reversing N6-methyladenosine (m6A) modifications on mRNAs, and it plays a crucial role in many physiological and pathological processes. Previous studies have shown that Alkbh5 is required for maintaining the function of leukemia stem cells but is dispensable for normal hematopoiesis. In this study, we found that Alkbh5 deletion led to a moderate increase in the number of multiple progenitor cell populations while compromising the long-term self-renewal capacity of hematopoietic stem cells (HSCs). Here, we used RNA-seq and m6A-seq strategies to explore the underlying molecular mechanism. At the molecular level, Alkbh5 may regulate hematopoiesis by reducing m6A modificationOpen medicine (Warsaw, Poland)20230766https://www.ebi.ac.uk/biostudies/studies/S-EPMC10426271biostudies-literatureAlkbh5 plays indispensable roles in maintaining self-renewal of hematopoietic stem cells.PMC10426271Liu YLiu ZLi ZZou LChen ZZhou CKuang MYang BXiao FShu YLiu SfalseAlkbh5 plays indispensable roles in maintaining self-renewal of hematopoietic stem cells.Alkbh5 is one of the primary demethylases responsible for reversing N6-methyladenosine (m6A) modifications on mRNAs, and it plays a crucial role in many physiological and pathological processes. Previous studies have shown that Alkbh5 is required for maintaining the function of leukemia stem cells but is dispensable for normal hematopoiesis. In this study, we found that Alkbh5 deletion led to a moderate increase in the number of multiple progenitor cell populations while compromising the long-term self-renewal capacity of hematopoietic stem cells (HSCs). Here, we used RNA-seq and m6A-seq strategies to explore the underlying molecular mechanism. At the molecular level, Alkbh5 may regulate hematopoiesis by reducing m6A modification2023-01-01T00:00:00Z20232025-04-19T06:49:38.821Z2025-04-19T06:49:38.821ZS-EPMC104262713758865610.1515/med-2023-0766