{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Valenzuela-Garcia LI"],"funding":["Consejo Nacional de Ciencia y Tecnología"],"pagination":["e0043223"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10433799"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["11(4)"],"pubmed_abstract":["A green fluorescent protein (GFP)-based whole-cell biosensor (WCB-GFP) for monitoring arsenic (As) was developed in Bacillus subtilis. To this end, we designed a reporter gene fusion carrying the <i>gfpmut3a</i> gene under the control of the promoter/operator region of the arsenic operon (<i>Pars::gfpmut3a</i>) in the extrachromosomal plasmid pAD123. This construct was transformed into B. subtilis 168, and the resultant strain was used as a whole-cell biosensor (<i>Bs</i>WCB-GFP) for the detection of As. The <i>Bs</i>WCB-GFP was specifically activated by inorganic As(III) and As(V), but not by dimethylarsinic acid [DMA(V)], and exhibited high tolerance to the noxious effects of arsenic. Accordingly, after 12 h exposure, B. subtilis cells carrying the <i>Pars::gfpmut3a</i> fusion exhibited "],"journal":["Microbiology spectrum"],"pubmed_title":["Design of a Whole-Cell Biosensor Based on Bacillus subtilis Spores and the Green Fluorescent Protein To Monitor Arsenic."],"pmcid":["PMC10433799"],"funding_grant_id":["A-1S-27116","321966"],"pubmed_authors":["Valenzuela-Garcia LI","Salas-Pacheco JM","Diaz-Valles JF","Alarcon-Herrera MT","Ayala-Garcia VM","Barraza-Salas M","Pedraza-Reyes M"],"additional_accession":[]},"is_claimable":false,"name":"Design of a Whole-Cell Biosensor Based on Bacillus subtilis Spores and the Green Fluorescent Protein To Monitor Arsenic.","description":"A green fluorescent protein (GFP)-based whole-cell biosensor (WCB-GFP) for monitoring arsenic (As) was developed in Bacillus subtilis. To this end, we designed a reporter gene fusion carrying the <i>gfpmut3a</i> gene under the control of the promoter/operator region of the arsenic operon (<i>Pars::gfpmut3a</i>) in the extrachromosomal plasmid pAD123. This construct was transformed into B. subtilis 168, and the resultant strain was used as a whole-cell biosensor (<i>Bs</i>WCB-GFP) for the detection of As. The <i>Bs</i>WCB-GFP was specifically activated by inorganic As(III) and As(V), but not by dimethylarsinic acid [DMA(V)], and exhibited high tolerance to the noxious effects of arsenic. Accordingly, after 12 h exposure, B. subtilis cells carrying the <i>Pars::gfpmut3a</i> fusion exhibited ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Aug","modification":"2026-07-15T00:42:25.645Z","creation":"2026-06-27T03:05:45.668Z"},"accession":"S-EPMC10433799","cross_references":{"pubmed":["37284752"],"doi":["10.1128/spectrum.00432-23"]}}