biostudies-literatureValenzuela-Garcia LIConsejo Nacional de Ciencia y Tecnologíae0043223https://www.ebi.ac.uk/biostudies/studies/S-EPMC10433799biostudies-literatureUnknown11(4)A green fluorescent protein (GFP)-based whole-cell biosensor (WCB-GFP) for monitoring arsenic (As) was developed in Bacillus subtilis. To this end, we designed a reporter gene fusion carrying the <i>gfpmut3a</i> gene under the control of the promoter/operator region of the arsenic operon (<i>Pars::gfpmut3a</i>) in the extrachromosomal plasmid pAD123. This construct was transformed into B. subtilis 168, and the resultant strain was used as a whole-cell biosensor (<i>Bs</i>WCB-GFP) for the detection of As. The <i>Bs</i>WCB-GFP was specifically activated by inorganic As(III) and As(V), but not by dimethylarsinic acid [DMA(V)], and exhibited high tolerance to the noxious effects of arsenic. Accordingly, after 12 h exposure, B. subtilis cells carrying the <i>Pars::gfpmut3a</i> fusion exhibited Microbiology spectrumDesign of a Whole-Cell Biosensor Based on Bacillus subtilis Spores and the Green Fluorescent Protein To Monitor Arsenic.PMC10433799A-1S-27116321966Valenzuela-Garcia LISalas-Pacheco JMDiaz-Valles JFAlarcon-Herrera MTAyala-Garcia VMBarraza-Salas MPedraza-Reyes MfalseDesign of a Whole-Cell Biosensor Based on Bacillus subtilis Spores and the Green Fluorescent Protein To Monitor Arsenic.A green fluorescent protein (GFP)-based whole-cell biosensor (WCB-GFP) for monitoring arsenic (As) was developed in Bacillus subtilis. To this end, we designed a reporter gene fusion carrying the <i>gfpmut3a</i> gene under the control of the promoter/operator region of the arsenic operon (<i>Pars::gfpmut3a</i>) in the extrachromosomal plasmid pAD123. This construct was transformed into B. subtilis 168, and the resultant strain was used as a whole-cell biosensor (<i>Bs</i>WCB-GFP) for the detection of As. The <i>Bs</i>WCB-GFP was specifically activated by inorganic As(III) and As(V), but not by dimethylarsinic acid [DMA(V)], and exhibited high tolerance to the noxious effects of arsenic. Accordingly, after 12 h exposure, B. subtilis cells carrying the <i>Pars::gfpmut3a</i> fusion exhibited 2023-01-01T00:00:00Z2023 Aug2026-07-15T00:42:25.645Z2026-06-27T03:05:45.668ZS-EPMC104337993728475210.1128/spectrum.00432-23