biostudies-literatureUnknown3(1)Alpuche-Lazcano SP<h4>Background</h4>As the COVID-19 pandemic continues to evolve, novel vaccines need to be developed that are readily manufacturable and provide clinical efficacy against emerging SARS-CoV-2 variants. Virus-like particles (VLPs) presenting the spike antigen at their surface offer remarkable benefits over other vaccine antigen formats; however, current SARS-CoV-2 VLP vaccines candidates in clinical development suffer from challenges including low volumetric productivity, poor spike antigen density, expression platform-driven divergent protein glycosylation and complex upstream/downstream processing requirements. Despite their extensive use for therapeutic protein manufacturing and proven ability to produce enveloped VLPs, Chinese Hamster Ovary (CHO) cells are rarely used for the commercial Communications medicine116https://www.ebi.ac.uk/biostudies/studies/S-EPMC10447459biostudies-literaturePreclinical evaluation of manufacturable SARS-CoV-2 spike virus-like particles produced in Chinese Hamster Ovary cells.PMC10447459Star ACass BDurocher YMaltais JSDudani RCui KCho JYBlouin JStuible MAlpuche-Lazcano SPTran AZoubchenok DDuque DMcCluskie MJHrapovic SHaqqani ARenner TMKelly JRobotham AAkache BWang XfalsePreclinical evaluation of manufacturable SARS-CoV-2 spike virus-like particles produced in Chinese Hamster Ovary cells.<h4>Background</h4>As the COVID-19 pandemic continues to evolve, novel vaccines need to be developed that are readily manufacturable and provide clinical efficacy against emerging SARS-CoV-2 variants. Virus-like particles (VLPs) presenting the spike antigen at their surface offer remarkable benefits over other vaccine antigen formats; however, current SARS-CoV-2 VLP vaccines candidates in clinical development suffer from challenges including low volumetric productivity, poor spike antigen density, expression platform-driven divergent protein glycosylation and complex upstream/downstream processing requirements. Despite their extensive use for therapeutic protein manufacturing and proven ability to produce enveloped VLPs, Chinese Hamster Ovary (CHO) cells are rarely used for the commercial 2023-01-01T00:00:00Z2023 Aug2026-04-29T02:25:46.383Z2025-02-19T04:01:44.253ZS-EPMC104474593761242310.1038/s43856-023-00340-7