{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sandberg TE"],"funding":["NNF Center for Biosustainability","Novo Nordisk Fonden","National Science Foundation"],"pagination":["107500"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10448532"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(9)"],"pubmed_abstract":["The bacterial strain JCVI-syn3.0 stands as the first example of a living organism with a minimized synthetic genome, derived from the <i>Mycoplasma mycoides</i> genome and chemically synthesized <i>in vitro</i>. Here, we report the experimental evolution of a syn3.0- derived strain. Ten independent replicates were evolved for several hundred generations, leading to growth rate improvements of > 15%. Endpoint strains possessed an average of 8 mutations composed of indels and SNPs, with a pronounced C/G- > A/T transversion bias. Multiple genes were repeated mutational targets across the independent lineages, including phase variable lipoprotein activation, 5 distinct; nonsynonymous substitutions in the same membrane transporter protein, and inactivation of an uncharacterized gene. Transcript"],"journal":["iScience"],"pubmed_title":["Adaptive evolution of a minimal organism with a synthetic genome."],"pmcid":["PMC10448532"],"funding_grant_id":["Biofoundry and Strain Design Teams","NNF10CC1016517","MCB 1840320","MCB 1818344","MCB 2221237","MCB 1840301"],"pubmed_authors":["Glass JI","Wise KS","Dalldorf C","Palsson BO","Szubin R","Sandberg TE","Feist AM"],"additional_accession":[]},"is_claimable":false,"name":"Adaptive evolution of a minimal organism with a synthetic genome.","description":"The bacterial strain JCVI-syn3.0 stands as the first example of a living organism with a minimized synthetic genome, derived from the <i>Mycoplasma mycoides</i> genome and chemically synthesized <i>in vitro</i>. Here, we report the experimental evolution of a syn3.0- derived strain. Ten independent replicates were evolved for several hundred generations, leading to growth rate improvements of > 15%. Endpoint strains possessed an average of 8 mutations composed of indels and SNPs, with a pronounced C/G- > A/T transversion bias. Multiple genes were repeated mutational targets across the independent lineages, including phase variable lipoprotein activation, 5 distinct; nonsynonymous substitutions in the same membrane transporter protein, and inactivation of an uncharacterized gene. Transcript","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Sep","modification":"2026-04-08T12:38:55.853Z","creation":"2025-04-21T15:02:35.052Z"},"accession":"S-EPMC10448532","cross_references":{"pubmed":["37636038"],"doi":["10.1016/j.isci.2023.107500"]}}