biostudies-literaturePedersen LThe Danish Veterinary and Food AdministrationThe Danish Milk Levy FundThe Danish Cattle Levy Fund2649https://www.ebi.ac.uk/biostudies/studies/S-EPMC10451256biostudies-literatureUnknown13(16)An increasing average herd size and complexity in farm structures call for a higher level of biosecurity. It can reduce the risk of introducing and establishing pathogens with multiple-pathway and indirect spread mechanisms, such as <i>Salmonella</i> Dublin, a pathogen with an increasing occurrence in dairy cattle farms across different countries and continents. Therefore, this study aimed to use existing knowledge to develop a framework with a supporting tool allowing for a time-efficient, yet comprehensive, assessment of biosecurity measures that can help prevent the introduction and establishment of <i>S.</i> Dublin in dairy herds. Based on the literature review, a seven-step biosecurity assessment framework was developed and evaluated in collaboration with biosecurity experts. The resuAnimals : an open access journal from MDPISemi-Quantitative Biosecurity Assessment Framework Targeting Prevention of the Introduction and Establishment of <i>Salmonella</i> Dublin in Dairy Cattle Herds.PMC10451256no numbers availableHoue HNielsen LRPedersen LRattenborg EfalseSemi-Quantitative Biosecurity Assessment Framework Targeting Prevention of the Introduction and Establishment of <i>Salmonella</i> Dublin in Dairy Cattle Herds.An increasing average herd size and complexity in farm structures call for a higher level of biosecurity. It can reduce the risk of introducing and establishing pathogens with multiple-pathway and indirect spread mechanisms, such as <i>Salmonella</i> Dublin, a pathogen with an increasing occurrence in dairy cattle farms across different countries and continents. Therefore, this study aimed to use existing knowledge to develop a framework with a supporting tool allowing for a time-efficient, yet comprehensive, assessment of biosecurity measures that can help prevent the introduction and establishment of <i>S.</i> Dublin in dairy herds. Based on the literature review, a seven-step biosecurity assessment framework was developed and evaluated in collaboration with biosecurity experts. The resu2023-01-01T00:00:00Z2023 Aug2025-04-18T18:48:36.95Z2025-04-07T06:29:46.477ZS-EPMC104512563762744010.3390/ani13162649