biostudies-literatureLong SNational Natural Science Foundation of China12534https://www.ebi.ac.uk/biostudies/studies/S-EPMC10454058biostudies-literatureUnknown24(16)<i>Hydrogenases catalyze the simple</i> yet important redox reaction between protons and electrons and H₂, thus mediating symbiotic interactions. The contribution of <i>hydrogenase</i> to this symbiosis and anti-oxidative damage was investigated using the <i>M. huakuii hypE</i> (encoding <i>hydrogenase</i> maturation protein) mutant. The <i>hypE</i> mutant grew a little faster than its parental 7653R and displayed decreased antioxidative capacity under H₂O₂-induced oxidative damage. Real-time quantitative PCR showed that <i>hypE</i> gene expression is significantly up-regulated in all the detected stages of nodule development. Although the <i>hypE</i> mutant can form nodules, the symbiotic ability was severely impaired, which led to an abnormal nodulation pInternational journal of molecular sciencesProteomic and Mutant Analysis of Hydrogenase Maturation Protein Gene <i>hypE</i> in Symbiotic Nitrogen Fixation of <i>Mesorhizobium huakuii</i>.PMC1045405831772399Su MYu FZou QChen XHu ACheng GLong SfalseProteomic and Mutant Analysis of Hydrogenase Maturation Protein Gene <i>hypE</i> in Symbiotic Nitrogen Fixation of <i>Mesorhizobium huakuii</i>.<i>Hydrogenases catalyze the simple</i> yet important redox reaction between protons and electrons and H₂, thus mediating symbiotic interactions. The contribution of <i>hydrogenase</i> to this symbiosis and anti-oxidative damage was investigated using the <i>M. huakuii hypE</i> (encoding <i>hydrogenase</i> maturation protein) mutant. The <i>hypE</i> mutant grew a little faster than its parental 7653R and displayed decreased antioxidative capacity under H₂O₂-induced oxidative damage. Real-time quantitative PCR showed that <i>hypE</i> gene expression is significantly up-regulated in all the detected stages of nodule development. Although the <i>hypE</i> mutant can form nodules, the symbiotic ability was severely impaired, which led to an abnormal nodulation p2023-01-01T00:00:00Z2023 Aug2025-05-18T13:41:48.546Z2025-05-18T13:41:48.546ZS-EPMC104540583762871510.3390/ijms241612534