{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Liu J"],"funding":["National Natural Science Foundation of China","International Science and Technology Cooperation Programme"],"pagination":["107495"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10457526"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["26(9)"],"pubmed_abstract":["Timely diagnosis of <i>Schistosoma</i> infection, particularly in the early stage is crucial for identifying infected hosts and then taking effective control strategies. Here, metagenomic next-generation sequencing was used to identify pathogen-specific circulating DNAs (cDNAs) in the sera/plasma of New Zealand rabbits infected with <i>S. japonicum</i>, and the identified cDNAs were validated by PCR and qPCR. Loop-mediated isothermal amplification (LAMP)-based CRISPR-Cas12a and recombinase polymerase amplification-based lateral flow strip (RPA-LF) methods combined with the newly identified cDNA were developed to evaluate the potentials for diagnosing murine and human schistosomiasis. The results indicated that twenty-two cDNAs were identified. The developed LAMP-based CRISPR/Cas12a and RPA"],"journal":["iScience"],"pubmed_title":["Metagenomic sequencing for identifying pathogen-specific circulating DNAs and development of diagnostic methods for schistosomiasis."],"pmcid":["PMC10457526"],"funding_grant_id":["CN202010594692.2","31672550","202310702322.X","2021YFE0191600"],"pubmed_authors":["Li X","Liu J","Li S","Giri BR","Wang X","Cheng G","Xia T","Sheng F"],"additional_accession":[]},"is_claimable":false,"name":"Metagenomic sequencing for identifying pathogen-specific circulating DNAs and development of diagnostic methods for schistosomiasis.","description":"Timely diagnosis of <i>Schistosoma</i> infection, particularly in the early stage is crucial for identifying infected hosts and then taking effective control strategies. Here, metagenomic next-generation sequencing was used to identify pathogen-specific circulating DNAs (cDNAs) in the sera/plasma of New Zealand rabbits infected with <i>S. japonicum</i>, and the identified cDNAs were validated by PCR and qPCR. Loop-mediated isothermal amplification (LAMP)-based CRISPR-Cas12a and recombinase polymerase amplification-based lateral flow strip (RPA-LF) methods combined with the newly identified cDNA were developed to evaluate the potentials for diagnosing murine and human schistosomiasis. The results indicated that twenty-two cDNAs were identified. The developed LAMP-based CRISPR/Cas12a and RPA","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Sep","modification":"2026-05-28T12:16:43.259Z","creation":"2025-04-07T10:20:20.625Z"},"accession":"S-EPMC10457526","cross_references":{"pubmed":["37636036"],"doi":["10.1016/j.isci.2023.107495"]}}