biostudies-literatureLiu JNational Natural Science Foundation of ChinaInternational Science and Technology Cooperation Programme107495https://www.ebi.ac.uk/biostudies/studies/S-EPMC10457526biostudies-literatureUnknown26(9)Timely diagnosis of <i>Schistosoma</i> infection, particularly in the early stage is crucial for identifying infected hosts and then taking effective control strategies. Here, metagenomic next-generation sequencing was used to identify pathogen-specific circulating DNAs (cDNAs) in the sera/plasma of New Zealand rabbits infected with <i>S. japonicum</i>, and the identified cDNAs were validated by PCR and qPCR. Loop-mediated isothermal amplification (LAMP)-based CRISPR-Cas12a and recombinase polymerase amplification-based lateral flow strip (RPA-LF) methods combined with the newly identified cDNA were developed to evaluate the potentials for diagnosing murine and human schistosomiasis. The results indicated that twenty-two cDNAs were identified. The developed LAMP-based CRISPR/Cas12a and RPAiScienceMetagenomic sequencing for identifying pathogen-specific circulating DNAs and development of diagnostic methods for schistosomiasis.PMC10457526CN202010594692.231672550202310702322.X2021YFE0191600Li XLiu JLi SGiri BRWang XCheng GXia TSheng FfalseMetagenomic sequencing for identifying pathogen-specific circulating DNAs and development of diagnostic methods for schistosomiasis.Timely diagnosis of <i>Schistosoma</i> infection, particularly in the early stage is crucial for identifying infected hosts and then taking effective control strategies. Here, metagenomic next-generation sequencing was used to identify pathogen-specific circulating DNAs (cDNAs) in the sera/plasma of New Zealand rabbits infected with <i>S. japonicum</i>, and the identified cDNAs were validated by PCR and qPCR. Loop-mediated isothermal amplification (LAMP)-based CRISPR-Cas12a and recombinase polymerase amplification-based lateral flow strip (RPA-LF) methods combined with the newly identified cDNA were developed to evaluate the potentials for diagnosing murine and human schistosomiasis. The results indicated that twenty-two cDNAs were identified. The developed LAMP-based CRISPR/Cas12a and RPA2023-01-01T00:00:00Z2023 Sep2026-05-28T12:16:43.259Z2025-04-07T10:20:20.625ZS-EPMC104575263763603610.1016/j.isci.2023.107495