biostudies-literatureKlafke KSão Paulo Research FoundationBrazilian Agricultural Research CorporationNational Council for Scientific and Technological Development1001https://www.ebi.ac.uk/biostudies/studies/S-EPMC10457946biostudies-literatureUnknown12(8)<i>Spodoptera frugiperda</i> (fall armyworm) is one of the most important maize pests in the world and the baculovirus <i>Spodoptera frugiperda multiple nucleopolyhedrovirus</i> (SfMNPV), a natural pathogen of this pest, has been used as a biopesticide for its control. At present, in vivo strategies at the commercial scale are employed by multiplying the virus in the host insect in biofactory facilities; however, in vitro large-scale production is an interesting alternative to overcome the limitations of baculoviruses massal production. This study aimed to develop the process of the SfMNPV in vitro production by evaluating the effects of different multiplicities of infection (MOI) and nutritional supplements, morphological and molecular analysis of the infection on the growth of Sf9 cells Pathogens (Basel, Switzerland)Bioreactor Production Process of <i>Spodoptera frugiperda multiple nucleopolyhedrovirus</i> Biopesticide.PMC10457946462343/2014-62018/08526-510.20.02.009.00.02.003 SEI2017/06371-1Klafke KSihler WTonso ASanches MMSouza MLfalseBioreactor Production Process of <i>Spodoptera frugiperda multiple nucleopolyhedrovirus</i> Biopesticide.<i>Spodoptera frugiperda</i> (fall armyworm) is one of the most important maize pests in the world and the baculovirus <i>Spodoptera frugiperda multiple nucleopolyhedrovirus</i> (SfMNPV), a natural pathogen of this pest, has been used as a biopesticide for its control. At present, in vivo strategies at the commercial scale are employed by multiplying the virus in the host insect in biofactory facilities; however, in vitro large-scale production is an interesting alternative to overcome the limitations of baculoviruses massal production. This study aimed to develop the process of the SfMNPV in vitro production by evaluating the effects of different multiplicities of infection (MOI) and nutritional supplements, morphological and molecular analysis of the infection on the growth of Sf9 cells 2023-01-01T00:00:00Z2023 Jul2025-04-27T00:16:09.793Z2025-04-06T17:48:15.957ZS-EPMC104579463762396110.3390/pathogens12081001