{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["15(8)"],"submitter":["Zahid M"],"funding":["NIH grants"],"pubmed_abstract":["Background: Amiodarone is underutilized due to significant off-target toxicities. We hypothesized that targeted delivery to the heart would lead to the lowering of the dose by utilizing a cardiomyocyte-targeting peptide (CTP), a cell-penetrating peptide identified by our prior phage display work. Methods: CTP was synthesized thiolated at the N-terminus, conjugated to amiodarone via Schiff base chemistry, HPLC purified, and confirmed with MALDI/TOF. The stability of the conjugate was assessed using serial HPLCs. Guinea pigs (GP) were injected intraperitoneally daily with vehicle (7 days), amiodarone (7 days; 80 mg/kg), CTP–amiodarone (5 days; 26.3 mg/kg), or CTP (5 days; 17.8 mg/kg), after which the GPs were euthanized, and the hearts were excised and perfused on a Langendorff apparatus wit"],"journal":["Pharmaceutics"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10459552"],"repository":["biostudies-literature"],"pubmed_title":["Cardiomyocyte-Targeting Peptide to Deliver Amiodarone"],"pmcid":["PMC10459552"],"funding_grant_id":["R41HL150908","R01HL153407"],"pubmed_authors":["Lopuszynski J","Agrawal V","Yagi H","Yurko R","Zahid M","Islam K","Salama G","Weber B"],"additional_accession":[]},"is_claimable":false,"name":"Cardiomyocyte-Targeting Peptide to Deliver Amiodarone","description":"Background: Amiodarone is underutilized due to significant off-target toxicities. We hypothesized that targeted delivery to the heart would lead to the lowering of the dose by utilizing a cardiomyocyte-targeting peptide (CTP), a cell-penetrating peptide identified by our prior phage display work. Methods: CTP was synthesized thiolated at the N-terminus, conjugated to amiodarone via Schiff base chemistry, HPLC purified, and confirmed with MALDI/TOF. The stability of the conjugate was assessed using serial HPLCs. Guinea pigs (GP) were injected intraperitoneally daily with vehicle (7 days), amiodarone (7 days; 80 mg/kg), CTP–amiodarone (5 days; 26.3 mg/kg), or CTP (5 days; 17.8 mg/kg), after which the GPs were euthanized, and the hearts were excised and perfused on a Langendorff apparatus wit","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Aug","modification":"2025-04-05T13:40:23.995Z","creation":"2025-04-05T13:40:23.995Z"},"accession":"S-EPMC10459552","cross_references":{}}