{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["222(11)"],"submitter":["Petsalaki E"],"funding":["Hellenic Foundation for Research and Innovation","Fondation Santé"],"pubmed_abstract":["In response to chromatin bridges, the abscission checkpoint delays completion of cytokinesis to prevent chromosome breakage or tetraploidization. Here, we show that spontaneous or replication stress-induced chromatin bridges exhibit \"knots\" of catenated and overtwisted DNA next to the midbody. Topoisomerase IIα (Top2α) forms abortive Top2-DNA cleavage complexes (Top2ccs) on DNA knots; furthermore, impaired Top2α-DNA cleavage activity correlates with chromatin bridge breakage in cytokinesis. Proteasomal degradation of Top2ccs is required for Rad17 localization to Top2-generated double-strand DNA ends on DNA knots; in turn, Rad17 promotes local recruitment of the MRN complex and downstream ATM-Chk2-INCENP signaling to delay abscission and prevent chromatin breakage. In contrast, dicentric ch"],"journal":["The Journal of cell biology"],"pagination":["e202303123"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10461104"],"repository":["biostudies-literature"],"pubmed_title":["The abscission checkpoint senses chromatin bridges through Top2α recruitment to DNA knots."],"pmcid":["PMC10461104"],"pubmed_authors":["Zachos G","Balafouti S","Kyriazi AA","Petsalaki E"],"additional_accession":[]},"is_claimable":false,"name":"The abscission checkpoint senses chromatin bridges through Top2α recruitment to DNA knots.","description":"In response to chromatin bridges, the abscission checkpoint delays completion of cytokinesis to prevent chromosome breakage or tetraploidization. Here, we show that spontaneous or replication stress-induced chromatin bridges exhibit \"knots\" of catenated and overtwisted DNA next to the midbody. Topoisomerase IIα (Top2α) forms abortive Top2-DNA cleavage complexes (Top2ccs) on DNA knots; furthermore, impaired Top2α-DNA cleavage activity correlates with chromatin bridge breakage in cytokinesis. Proteasomal degradation of Top2ccs is required for Rad17 localization to Top2-generated double-strand DNA ends on DNA knots; in turn, Rad17 promotes local recruitment of the MRN complex and downstream ATM-Chk2-INCENP signaling to delay abscission and prevent chromatin breakage. In contrast, dicentric ch","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Nov","modification":"2026-07-14T23:16:19.468Z","creation":"2025-04-06T12:47:52.536Z"},"accession":"S-EPMC10461104","cross_references":{"pubmed":["37638884"],"doi":["10.1083/jcb.202303123"]}}