biostudies-literatureChen JNatural Science Foundation of Xinjiang Uygur Autonomous RegionNatural Science Foundation of Hunan ProvinceChina Postdoctoral Science FoundationNational Natural Science Foundation of China295https://www.ebi.ac.uk/biostudies/studies/S-EPMC10464265biostudies-literatureUnknown21(1)<h4>Background</h4>Acute lung injury (ALI), manifested as strong pulmonary inflammation and alveolar epithelial damage, is a life-threatening disease with high morbidity and mortality. Small extracellular vesicles (sEVs), secreted by multiple types of cells, are critical cellular communication mediators and can inhibit inflammation by transferring bioactive molecules, such as microRNAs (miRNAs). Thus, we hypothesized that sEVs derived from mesenchymal stromal cells (MSC sEVs) could transfer miRNAs to attenuate inflammation of lung epithelial cells during ALI.<h4>Methods</h4>C57BL/6 male mice were intratracheally administered LPS (10 mg/kg). Six hours later, the mice were randomly administered with MSC sEVs (40 µg per mouse in 150 µl of saline), which were collected by ultracentrifugation. Journal of nanobiotechnologyHuman umbilical cord mesenchymal stromal cell small extracellular vesicle transfer of microRNA-223-3p to lung epithelial cells attenuates inflammation in acute lung injury in mice.PMC10464265816000632022M7135202022D01C4982017JJ3484Yang HNiu RLi YMa SChen JZhu FPan PHao HLuo BZhang PfalseHuman umbilical cord mesenchymal stromal cell small extracellular vesicle transfer of microRNA-223-3p to lung epithelial cells attenuates inflammation in acute lung injury in mice.<h4>Background</h4>Acute lung injury (ALI), manifested as strong pulmonary inflammation and alveolar epithelial damage, is a life-threatening disease with high morbidity and mortality. Small extracellular vesicles (sEVs), secreted by multiple types of cells, are critical cellular communication mediators and can inhibit inflammation by transferring bioactive molecules, such as microRNAs (miRNAs). Thus, we hypothesized that sEVs derived from mesenchymal stromal cells (MSC sEVs) could transfer miRNAs to attenuate inflammation of lung epithelial cells during ALI.<h4>Methods</h4>C57BL/6 male mice were intratracheally administered LPS (10 mg/kg). Six hours later, the mice were randomly administered with MSC sEVs (40 µg per mouse in 150 µl of saline), which were collected by ultracentrifugation. 2023-01-01T00:00:00Z2023 Aug2025-04-26T14:39:10.717Z2025-04-06T14:37:42.994ZS-EPMC104642653762640810.1186/s12951-023-02038-3