{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Shahriari Felordi M"],"funding":["Royan Institute","Bahar Tashkhis Teb Co"],"pagination":["2572-2582"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10468655"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["27(17)"],"pubmed_abstract":["Anti-cancer properties of (-)-epigallocatechin-3-gallate (EGCG) are mediated via apoptosis induction, as well as inhibition of cell proliferation and histone deacetylase. Accumulation of stabilized cellular FLICE-inhibitory protein (c-FLIP)/Ku70 complex in the cytoplasm inhibits apoptosis through interruption of extrinsic apoptosis pathway. In this study, we evaluated the anti-cancer role of EGCG in gastric cancer (GC) cells through dissociation of c-FLIP/Ku70 complex. MKN-45 cells were treated with EGCG or its antagonist MG149 for 24 h. Apoptosis was evaluated by flow cytometry and quantitative RT-PCR. Protein expression of c-FLIP and Ku70 was analysed using western blot and immunofluorescence. Dissociation of c-FLIP/Ku70 complex as well as Ku70 translocation were studied by sub-cellular "],"journal":["Journal of cellular and molecular medicine"],"pubmed_title":["(-)-Epigallocatechin-3-gallate induced apoptosis by dissociation of c-FLIP/Ku70 complex in gastric cancer cells."],"pmcid":["PMC10468655"],"funding_grant_id":["R.98005","R1398-0335","R1398‐0335"],"pubmed_authors":["Shahriari Felordi M","Alikhani M","Piryaei A","Ebrahimi M","Aboulkheyr Es H","Farzaneh Z","Alipour Choshali M","Vosough M","Najimi M"],"additional_accession":[]},"is_claimable":false,"name":"(-)-Epigallocatechin-3-gallate induced apoptosis by dissociation of c-FLIP/Ku70 complex in gastric cancer cells.","description":"Anti-cancer properties of (-)-epigallocatechin-3-gallate (EGCG) are mediated via apoptosis induction, as well as inhibition of cell proliferation and histone deacetylase. Accumulation of stabilized cellular FLICE-inhibitory protein (c-FLIP)/Ku70 complex in the cytoplasm inhibits apoptosis through interruption of extrinsic apoptosis pathway. In this study, we evaluated the anti-cancer role of EGCG in gastric cancer (GC) cells through dissociation of c-FLIP/Ku70 complex. MKN-45 cells were treated with EGCG or its antagonist MG149 for 24 h. Apoptosis was evaluated by flow cytometry and quantitative RT-PCR. Protein expression of c-FLIP and Ku70 was analysed using western blot and immunofluorescence. Dissociation of c-FLIP/Ku70 complex as well as Ku70 translocation were studied by sub-cellular ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Sep","modification":"2025-04-25T20:22:19.981Z","creation":"2025-04-06T08:18:26.779Z"},"accession":"S-EPMC10468655","cross_references":{"pubmed":["37537749"],"doi":["10.1111/jcmm.17873"]}}