{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["12(15)"],"submitter":["Wang C"],"pubmed_abstract":["<h4>Background</h4>Breast cancer (BC) is the leading cause of cancer-related death among women. One of the hallmarks of cancer is sustained angiogenesis. YAP/STAT3 may promote angiogenesis and driving BC progression. This study aimed to investigate how YAP/STAT3 affects the immune microenvironment in BC and understand the underlying mechanism.<h4>Methods</h4>To establish a tumor-associated macrophages (TAMs) model, macrophages were cultured in the 4T1 cell culture medium. A BC mouse model was created by injecting 4T1 cells. The expression of YAP, STAT3, p-STAT3, VEGF, VEGFR-2, and PD-L1 was analyzed using immunofluorescence, western blotting, and quantitative real-time PCR. Flow cytometry was used to identify M1 and M2 macrophages, CD4<sup>+</sup> T, CD8<sup>+</sup> T, and Treg cells. Leve"],"journal":["Cancer medicine"],"pagination":["16295-16309"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10469732"],"repository":["biostudies-literature"],"pubmed_title":["YAP/STAT3 inhibited CD8<sup>+</sup> T cells activity in the breast cancer immune microenvironment by inducing M2 polarization of tumor-associated macrophages."],"pmcid":["PMC10469732"],"pubmed_authors":["He S","Tan X","Wang C","Shen N","Guo Q"],"additional_accession":[]},"is_claimable":false,"name":"YAP/STAT3 inhibited CD8<sup>+</sup> T cells activity in the breast cancer immune microenvironment by inducing M2 polarization of tumor-associated macrophages.","description":"<h4>Background</h4>Breast cancer (BC) is the leading cause of cancer-related death among women. One of the hallmarks of cancer is sustained angiogenesis. YAP/STAT3 may promote angiogenesis and driving BC progression. This study aimed to investigate how YAP/STAT3 affects the immune microenvironment in BC and understand the underlying mechanism.<h4>Methods</h4>To establish a tumor-associated macrophages (TAMs) model, macrophages were cultured in the 4T1 cell culture medium. A BC mouse model was created by injecting 4T1 cells. The expression of YAP, STAT3, p-STAT3, VEGF, VEGFR-2, and PD-L1 was analyzed using immunofluorescence, western blotting, and quantitative real-time PCR. Flow cytometry was used to identify M1 and M2 macrophages, CD4<sup>+</sup> T, CD8<sup>+</sup> T, and Treg cells. Leve","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Aug","modification":"2025-04-19T20:04:26.175Z","creation":"2025-04-19T20:04:26.175Z"},"accession":"S-EPMC10469732","cross_references":{"pubmed":["37329188"],"doi":["10.1002/cam4.6242"]}}