{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["76"],"submitter":["Bai Z"],"funding":["Ministry of Science and Technology of the People&amp;apos;s Republic of China"],"pubmed_abstract":["<h4>Objective</h4>Peroxiredoxin 1 (PRDX1) is a peroxidase and guards against oxidative stress by scavenging intracellular peroxides, whereas it also has been shown to stimulate inflammatory response by functioning as a chaperone protein. The potential in vivo link between PRDX1's peroxidase activity and its pro-inflammatory activity remains elusive.<h4>Methods</h4>We generated peroxidase-dead PRDX1 variant mice by mutating its peroxidatic cysteine at 52 (Cys52) to serine, here referred to as PRDX1<sup>Cys52Ser</sup>. Trx-TrxR-NADPH coupled activity assay was applied to evaluate the peroxidase activity of global PRDX in PRDX1<sup>Cys52Ser</sup> variant mice. PRDX1<sup>Cys52Ser</sup> mice and their wild-type littermates were subjected to western diet or methionine and choline deficient diet "],"journal":["Molecular metabolism"],"pagination":["101789"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC10470253"],"repository":["biostudies-literature"],"pubmed_title":["PRDX1 Cys52Ser variant alleviates nonalcoholic steatohepatitis by reducing inflammation in mice."],"pmcid":["PMC10470253"],"pubmed_authors":["Yin W","Tang M","Xie X","Liu R","Bai Z","Luo C","Shi X"],"additional_accession":[]},"is_claimable":false,"name":"PRDX1 Cys52Ser variant alleviates nonalcoholic steatohepatitis by reducing inflammation in mice.","description":"<h4>Objective</h4>Peroxiredoxin 1 (PRDX1) is a peroxidase and guards against oxidative stress by scavenging intracellular peroxides, whereas it also has been shown to stimulate inflammatory response by functioning as a chaperone protein. The potential in vivo link between PRDX1's peroxidase activity and its pro-inflammatory activity remains elusive.<h4>Methods</h4>We generated peroxidase-dead PRDX1 variant mice by mutating its peroxidatic cysteine at 52 (Cys52) to serine, here referred to as PRDX1<sup>Cys52Ser</sup>. Trx-TrxR-NADPH coupled activity assay was applied to evaluate the peroxidase activity of global PRDX in PRDX1<sup>Cys52Ser</sup> variant mice. PRDX1<sup>Cys52Ser</sup> mice and their wild-type littermates were subjected to western diet or methionine and choline deficient diet ","dates":{"release":"2023-01-01T00:00:00Z","publication":"2023 Aug","modification":"2025-04-18T18:51:58.308Z","creation":"2025-04-07T06:33:51.724Z"},"accession":"S-EPMC10470253","cross_references":{"pubmed":["37562742"],"doi":["10.1016/j.molmet.2023.101789"]}}