biostudies-literatureUra TJapan Society for the Promotion of ScienceNew Energy and Industrial Technology Development Organization14381https://www.ebi.ac.uk/biostudies/studies/S-EPMC10474089biostudies-literatureUnknown13(1)Biological properties of protein molecules depend on their interaction with other molecules, and enzymes are no exception. Enzyme activities are controlled by their interaction with other molecules in living cells. Enzyme activation and their catalytic properties in the presence of different types of polymers have been studied in vitro, although these studies are restricted to only a few enzymes. In this study, we show that addition of poly-l-lysine (PLL) can increase the enzymatic activity of multiple oxidoreductases through formation of enzyme assemblies. Oxidoreductases with an overall negative charge, such as l-lactate oxidase, d-lactate dehydrogenase, pyruvate oxidase, and acetaldehyde dehydrogenase, each formed assemblies with the positively charged PLL via electrostatic interactionsScientific reportsActivation of oxidoreductases by the formation of enzyme assembly.PMC10474089JPNP1400422H0543720J21734Shiraki KTamada TUra TTakakusagi YSakakibara NMikawa THirano YfalseActivation of oxidoreductases by the formation of enzyme assembly.Biological properties of protein molecules depend on their interaction with other molecules, and enzymes are no exception. Enzyme activities are controlled by their interaction with other molecules in living cells. Enzyme activation and their catalytic properties in the presence of different types of polymers have been studied in vitro, although these studies are restricted to only a few enzymes. In this study, we show that addition of poly-l-lysine (PLL) can increase the enzymatic activity of multiple oxidoreductases through formation of enzyme assemblies. Oxidoreductases with an overall negative charge, such as l-lactate oxidase, d-lactate dehydrogenase, pyruvate oxidase, and acetaldehyde dehydrogenase, each formed assemblies with the positively charged PLL via electrostatic interactions2023-01-01T00:00:00Z2023 Sep2025-04-25T18:41:10.529Z2024-10-19T06:20:16.171ZS-EPMC104740893765812910.1038/s41598-023-41789-9