biostudies-literatureKroidl IUniversity Hospital, LMU MunichUniversitätsklinik MünchenEuroimmun and Roche provided kits and machines for analyses at discounted rates for the studyBavarian State Ministry of Science and the ArtsEuropean-wide Consortium ORCHESTRAGerman Ministry for Education and Research200https://www.ebi.ac.uk/biostudies/studies/S-EPMC10474769biostudies-literatureUnknown20(1)<h4>Background</h4>Measuring specific anti-SARS-CoV-2 antibodies has become one of the main epidemiological tools to survey the ongoing SARS-CoV-2 pandemic, but also vaccination response. The WHO made available a set of well-characterized samples derived from recovered individuals to allow normalization between different quantitative anti-Spike assays to defined Binding Antibody Units (BAU).<h4>Methods</h4>To assess sero-responses longitudinally, a cohort of ninety-nine SARS-CoV-2 RT-PCR positive subjects was followed up together with forty-five vaccinees without previous infection but with two vaccinations. Sero-responses were evaluated using a total of six different assays: four measuring anti-Spike proteins (converted to BAU), one measuring anti-Nucleocapsid proteins and one SARS-CoV-2 Virology journalStudying temporal titre evolution of commercial SARS-CoV-2 assays reveals significant shortcomings of using BAU standardization for comparison.PMC1047476901KI20271101016167Weigl NKeßler ALeidl RGasser MSchandelmaier PPuchinger KThiel VMayrhofer RDurner JGolschan EBohnlein RKroidl IStruber LNoller JMGHaselwarter TWustrow PHuber KPritsch MKroidl AStruber AGutierrez PCaroli FBitzer PGuglielmini EMSedlmeier JAlamoudi EHerrmann MBednarski FBaldassarre VFischer SMarkgraf AZielke AFingerle VWolfl RJanke CPlank MSchafer NLapteva EGari MColeman JDo AEser TGauder SBeyerl JFrese JRubio-Acero RZimmermann TDeak FHorn SZeggini ELaxy MEberle UGeisenberger ORadon KDiepers PCastelletti NDobler GNorena ISchluse BThiesbrummel SHillmann SBaumann MPleimelding CHillari LHalfmann CTang JWallrauch CBritz VLe Gleut RStrobl KNikolaides SSchneider ARaimundez EGrauvogl VLiedl LThur NEckstein JKresin JLucaj XHinske CDiekmannshemke JGeier RLuppa FMetaxa DVon Lovenberg EBakuli AFensterseifer HTheis FNafziger ASZuche LKiani CRaschka JMaczka ASchneider LGezgin KHeiber AKreider NRuci VContento LThumser EHofinger MHofmann LSchalte YGilberg LDiefenbach MNWieser ALucke ENiermeyer KAhmed MIMVon Lovenberg JMuller KPruckner SLang MSchunk MMekota AMPoll VBrauer APletschette MHoelscher MLang CPattard LReinkemeyer CUllrich JMatcau PFeyereisen MBruger JFroschl GWaibel JLange SWolff JZimmer TCzwienzek AGillig KHernandez ABunz FHofberger TNascimento NHartinger LHaderer AKlugherz IRothe CVos CZange SEser TMLindner FMang PKappl UFalk PMuller HBrand IBehlen MBecker MMohr EPusl KRiess FPaunovic IKhan ZGirl PHoefflin JKunder PReich JSoler ASchultz SGeldmacher CThomschke AGuggenbuhl JWurfel TKarger LHasenauer JHeller LSothmann PWinter SWullinger PSchwettmann LSintu-Sempta LSaathoff EKoCo19/ORCHESTRA Study GroupAnderson JPutz POlbrich LForster FHannes MBemirayev OVollmayr VFuchs CfalseStudying temporal titre evolution of commercial SARS-CoV-2 assays reveals significant shortcomings of using BAU standardization for comparison.<h4>Background</h4>Measuring specific anti-SARS-CoV-2 antibodies has become one of the main epidemiological tools to survey the ongoing SARS-CoV-2 pandemic, but also vaccination response. The WHO made available a set of well-characterized samples derived from recovered individuals to allow normalization between different quantitative anti-Spike assays to defined Binding Antibody Units (BAU).<h4>Methods</h4>To assess sero-responses longitudinally, a cohort of ninety-nine SARS-CoV-2 RT-PCR positive subjects was followed up together with forty-five vaccinees without previous infection but with two vaccinations. Sero-responses were evaluated using a total of six different assays: four measuring anti-Spike proteins (converted to BAU), one measuring anti-Nucleocapsid proteins and one SARS-CoV-2 2023-01-01T00:00:00Z2023 Sep2025-04-18T18:48:44.405Z2024-11-08T22:53:52.194ZS-EPMC104747693765845410.1186/s12985-023-02167-z